Co-localization of a CD1d-binding glycolipid with an adenovirus-based malaria vaccine for a potent adjuvant effect

Xiangming Li, Jing Huang, Akira Kawamura, Ryota Funakoshi, Steven A. Porcelli, Moriya Tsuji

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A CD1d-binding, invariant (i) natural killer T (NKT)-cell stimulatory glycolipid, α-Galactosylceramide (αGalCer), has been shown to act as an adjuvant. We previously identified a fluorinated phenyl ring-modified αGalCer analog, 7DW8-5, displaying a higher binding affinity for CD1d molecule and more potent adjuvant activity than αGalCer. In the present study, 7DW8-5 co-administered intramuscularly (i.m.) with a recombinant adenovirus expressing a Plasmodium yoelii circumsporozoite protein (PyCSP), AdPyCS, has led to a co-localization of 7DW8-5 and a PyCSP in draining lymph nodes (dLNs), particularly in dendritic cells (DCs). This occurrence initiates a cascade of events, such as the recruitment of DCs to dLNs and their activation and maturation, and the enhancement of the ability of DCs to prime CD8+ T cells induced by AdPyCS and ultimately leading to a potent adjuvant effect and protection against malaria.

Original languageEnglish (US)
Pages (from-to)3171-3177
Number of pages7
JournalVaccine
Volume35
Issue number24
DOIs
StatePublished - May 31 2017

Fingerprint

Galactosylceramides
Malaria Vaccines
vaccine adjuvants
glycolipids
Glycolipids
Adenoviridae
dendritic cells
Plasmodium yoelii
Dendritic Cells
adjuvants
lymph nodes
CD1d Antigen
T-lymphocytes
Lymph Nodes
Natural Killer T-Cells
natural killer cells
malaria
Malaria
proteins
T-Lymphocytes

Keywords

  • Adenovirus
  • CD1d
  • CD8+ T cells
  • Co-localization
  • Dendritic cells
  • Glycolipid adjuvant
  • Intramuscular injection
  • Lymph node
  • Malaria vaccine
  • Protection

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Co-localization of a CD1d-binding glycolipid with an adenovirus-based malaria vaccine for a potent adjuvant effect. / Li, Xiangming; Huang, Jing; Kawamura, Akira; Funakoshi, Ryota; Porcelli, Steven A.; Tsuji, Moriya.

In: Vaccine, Vol. 35, No. 24, 31.05.2017, p. 3171-3177.

Research output: Contribution to journalArticle

Li, Xiangming ; Huang, Jing ; Kawamura, Akira ; Funakoshi, Ryota ; Porcelli, Steven A. ; Tsuji, Moriya. / Co-localization of a CD1d-binding glycolipid with an adenovirus-based malaria vaccine for a potent adjuvant effect. In: Vaccine. 2017 ; Vol. 35, No. 24. pp. 3171-3177.
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