Co-expression of GPR30 and ERβ and their association with disease progression in uterine carcinosarcoma

Gloria S. Huang, Marc J. Gunter, Rebecca C. Arend, Maomi Li, Hugo Arias-Pulido, Eric R. Prossnitz, Gary L. Goldberg, Harriet O. Smith

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: We sought to evaluate the expression of G protein-coupled receptor 30 (GPR30) and estrogen receptor (ER)β in uterine carcinosarcoma (CS). Study Design: Immunohistochemistry was performed using antibodies to GPR30, ERβ, ERα, and progesterone receptor (PR). The staining intensity and percentage of positive cells were scored for each tissue section. Expression levels were compared using the Wilcoxon rank sum test. Correlation was evaluated by Spearman rho and logistic regression. Results: Compared with normal endometrium, CS had lower ERα and PR expression (both P < .01) but higher GPR30 epithelial expression (P = .03). Advanced-stage CS had higher GPR30 (P < .01) and ERβ (P = .02) epithelial expression compared with early-stage CS. Expression of GPR30 and ERβ correlated with each other (P < .01), and not with ERα or PR. Conclusion: In uterine CS, GPR30 and ERβ are coordinately overexpressed and expression levels increase in advanced-stage disease, supporting the involvement of alternative ERs in disease progression.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume203
Issue number3
DOIs
StatePublished - Sep 2010

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Carcinosarcoma
G-Protein-Coupled Receptors
Estrogen Receptors
Disease Progression
Progesterone Receptors
Nonparametric Statistics
Endometrium
Logistic Models
Immunohistochemistry
Staining and Labeling
Antibodies

Keywords

  • estrogen receptor
  • G protein-coupled receptor 30
  • uterine carcinosarcoma

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Co-expression of GPR30 and ERβ and their association with disease progression in uterine carcinosarcoma. / Huang, Gloria S.; Gunter, Marc J.; Arend, Rebecca C.; Li, Maomi; Arias-Pulido, Hugo; Prossnitz, Eric R.; Goldberg, Gary L.; Smith, Harriet O.

In: American Journal of Obstetrics and Gynecology, Vol. 203, No. 3, 09.2010.

Research output: Contribution to journalArticle

Huang, Gloria S. ; Gunter, Marc J. ; Arend, Rebecca C. ; Li, Maomi ; Arias-Pulido, Hugo ; Prossnitz, Eric R. ; Goldberg, Gary L. ; Smith, Harriet O. / Co-expression of GPR30 and ERβ and their association with disease progression in uterine carcinosarcoma. In: American Journal of Obstetrics and Gynecology. 2010 ; Vol. 203, No. 3.
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