Clustering class I MHC modulates sensitivity of T cell recognition

David R. Fooksman, Gigi Kwik Grönvall, Qing Tang, Michael Edidin

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

T cell recognition of peptide-MHC is highly specific and is sensitive to very low levels of agonist peptide; however, it is unclear how this effect is achieved or regulated. In this study we show that clustering class I MHC molecules on the cell surface of B lymphoblasts enhances their recognition by mouse and human T cells. We increased clustering of MHC I molecules by two methods, cholesterol depletion and direct cross-linking of a dimerizable MHC construct. Imaging showed that both treatments increased the size and intensity of MHC clusters on the cell surface. Enlarged clusters correlated with enhanced lysis and T cell effector function. Enhancements were peptide-specific and greatest at low concentrations of peptide. Clustering MHC class I enhanced recognition of both strong and weak agonists but not null peptide. Our results indicate that the lateral organization of MHC class I on the cell surface can modulate the sensitivity of T cell recognition of agonist peptide.

Original languageEnglish (US)
Pages (from-to)6673-6680
Number of pages8
JournalJournal of Immunology
Volume176
Issue number11
StatePublished - Jun 1 2006
Externally publishedYes

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Cluster Analysis
T-Lymphocytes
Peptides
Cholesterol

ASJC Scopus subject areas

  • Immunology

Cite this

Fooksman, D. R., Grönvall, G. K., Tang, Q., & Edidin, M. (2006). Clustering class I MHC modulates sensitivity of T cell recognition. Journal of Immunology, 176(11), 6673-6680.

Clustering class I MHC modulates sensitivity of T cell recognition. / Fooksman, David R.; Grönvall, Gigi Kwik; Tang, Qing; Edidin, Michael.

In: Journal of Immunology, Vol. 176, No. 11, 01.06.2006, p. 6673-6680.

Research output: Contribution to journalArticle

Fooksman, DR, Grönvall, GK, Tang, Q & Edidin, M 2006, 'Clustering class I MHC modulates sensitivity of T cell recognition', Journal of Immunology, vol. 176, no. 11, pp. 6673-6680.
Fooksman DR, Grönvall GK, Tang Q, Edidin M. Clustering class I MHC modulates sensitivity of T cell recognition. Journal of Immunology. 2006 Jun 1;176(11):6673-6680.
Fooksman, David R. ; Grönvall, Gigi Kwik ; Tang, Qing ; Edidin, Michael. / Clustering class I MHC modulates sensitivity of T cell recognition. In: Journal of Immunology. 2006 ; Vol. 176, No. 11. pp. 6673-6680.
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