Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis

Research output: Contribution to journalArticle

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Abstract

Diaminopimelate (DAP) is used by bacteria for the synthesis of lysine. In many species of bacteria, including mycobacteria, DAP is also used for peptidoglycan biosynthesis. In this report we describe the cloning of the dapB gene encoding dihydrodipicolinate reductase (DHPR), which catalyzes a key branch point reaction in the bacterial DAP biosynthetic pathway, from Mycobacterium tuberculosis. Analyses of the DapB proteins from different bacterial species suggest that two different classes of DHPR enzymes may exist in bacteria.

Original languageEnglish (US)
Pages (from-to)2777-2782
Number of pages6
JournalJournal of Bacteriology
Volume179
Issue number8
StatePublished - 1997

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Dihydrodipicolinate Reductase
Mycobacterium tuberculosis
Organism Cloning
Bacteria
Genes
Bacterial Proteins
Peptidoglycan
Biosynthetic Pathways
Mycobacterium
Lysine
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis. / Pavelka, Martin S.; Weisbrod, Torin R.; Jacobs, William R.

In: Journal of Bacteriology, Vol. 179, No. 8, 1997, p. 2777-2782.

Research output: Contribution to journalArticle

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