Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor

Andreas Jenny, Walter Keller

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

3′-processing of mRNA precursors depends on several protein factors. One of them, cleavage and polyadenylation specificity factor (CPSF) is required for the cleavage of the mRNA precursor as well as for the tail elongation reaction. We have obtained complementary DNA encoding the 160 kDa subunit, which had previously been shown to interact with the AAUAAA polyadenylation signal. The cDNAs code for an open reading frame of 1444 amino acids. The translated protein has a calculated molecular weight of 161 kDa and a predicted pl of 6.2. Polyclonal antibodies raised against a bacterially expressed fragment of the cDNA recognise the 160 kDa subunit of purified calf thymus CPSF. The sequence contains a possible nuclear localisation signal but none of the known RNA binding motifs. It does, however, show sequence similarities to a UV-damaged DNA binding protein (UVdDb).

Original languageEnglish (US)
Pages (from-to)2629-2635
Number of pages7
JournalNucleic Acids Research
Volume23
Issue number14
DOIs
StatePublished - Jul 25 1995
Externally publishedYes

Fingerprint

Cleavage And Polyadenylation Specificity Factor
Cloning
CDNA
Specificity
Organism Cloning
Encoding
DNA
Complementary DNA
RNA Precursors
Proteins
Thymus
Precursor
Messenger RNA
RNA
Antibodies
Protein
Amino acids
Elongation
DNA-binding Protein
Nuclear Localization Signals

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor. / Jenny, Andreas; Keller, Walter.

In: Nucleic Acids Research, Vol. 23, No. 14, 25.07.1995, p. 2629-2635.

Research output: Contribution to journalArticle

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