Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase

M. A. O'Connell, S. Krause, M. Higuchi, J. J. Hsuan, N. F. Totty, Andreas Jenny, W. Keller

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

Double-stranded RNA (dsRNA)-specific adenosine deaminase converts adenosine to inosine in dsRNA. The protein has been purified from calf thymus, and here we describe the cloning of cDNAs encoding both the human and rat proteins as well as a partial bovine clone. The human and rat clones are very similar at the amino acid level except at their N termini and contain three dsRNA binding motifs, a putative nuclear targeting signal, and a possible deaminase motif. Antibodies raised against the protein encoded by the partial bovine clone specifically recognize the calf thymus dsRNA adenosine deaminase. Furthermore, the antibodies can immunodeplete a calf thymus extract of dsRNA adenosine deaminase activity, and the activity can be restored by addition of pure bovine deaminase. Staining of HeLa cells confirms the nuclear localization of the dsRNA-specific adenosine deaminase. In situ hybridization in rat brain slices indicates a widespread distribution of the enzyme in the brain.

Original languageEnglish (US)
Pages (from-to)1389-1397
Number of pages9
JournalMolecular and Cellular Biology
Volume15
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

Adenosine Deaminase
Double-Stranded RNA
Organism Cloning
Complementary DNA
Clone Cells
Thymus Gland
Thymus Extracts
Inosine
Proteins
Antibodies
Brain
HeLa Cells
Adenosine
In Situ Hybridization
Staining and Labeling
Amino Acids
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

O'Connell, M. A., Krause, S., Higuchi, M., Hsuan, J. J., Totty, N. F., Jenny, A., & Keller, W. (1995). Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase. Molecular and Cellular Biology, 15(3), 1389-1397.

Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase. / O'Connell, M. A.; Krause, S.; Higuchi, M.; Hsuan, J. J.; Totty, N. F.; Jenny, Andreas; Keller, W.

In: Molecular and Cellular Biology, Vol. 15, No. 3, 1995, p. 1389-1397.

Research output: Contribution to journalArticle

O'Connell, MA, Krause, S, Higuchi, M, Hsuan, JJ, Totty, NF, Jenny, A & Keller, W 1995, 'Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase', Molecular and Cellular Biology, vol. 15, no. 3, pp. 1389-1397.
O'Connell MA, Krause S, Higuchi M, Hsuan JJ, Totty NF, Jenny A et al. Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase. Molecular and Cellular Biology. 1995;15(3):1389-1397.
O'Connell, M. A. ; Krause, S. ; Higuchi, M. ; Hsuan, J. J. ; Totty, N. F. ; Jenny, Andreas ; Keller, W. / Cloning of cDNAs encoding mammalian double-stranded RNA-specific adenosine deaminase. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 3. pp. 1389-1397.
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