Cloning of bcl-6, the locus involved in chromosome translocations affecting band 3q27 in B-cell lymphoma

B. Hilda Ye, P. H. Rao, R. S K Chaganti, Riccardo Dalla-Favera

Research output: Contribution to journalArticle

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Abstract

Chromosomal translocations involving band 3q27 and various chromosomal sites, including the sites of the immunoglobulin (Ig) loci (14q32, 2pl2, 22q11), represent recurrent aberrations in non-Hodgkin's lymphoma (NHL). In order to identify the putative protooncogene involved in these translocations, we have cloned the breakpoints from two B-cell NHL cases carrying t(3;14)(q27;q32) translocations by screening genomic DNA libraries constructed from NHL biopsy samples with immunoglobulin probes. Several recombinant phages have been obtained from each case and shown to contain sequences from both 14q32 and 3q27 by fluorescence in situ hybridization mapping on metaphase chromosomes. In both cases, the translocation breakpoints were found within the switch region of the Ig heavy-chain locus on 14q32 and within the same 3-kilobase region on 3q27. When used in Southern blot hybridization, a probe from the 3q27 region detected rearrangements in an additional five NHL cases carrying 3q27 translocations with 14q32 or other genomic sites. The same probe detected a predominant 2.4-kilobase mRNA species in several lymphoid cell lines analyzed by Northern blot hybridization. These data suggest that chromosomal breakpoints in 3q27 cluster in the proximity of a transcribed gene which represents a candidate protooncogene (bcl-6) involved in B-cell NHL pathogenesis.

Original languageEnglish (US)
Pages (from-to)2732-2735
Number of pages4
JournalCancer Research
Volume53
Issue number12
StatePublished - Jun 15 1993
Externally publishedYes

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B-Cell Lymphoma
Non-Hodgkin's Lymphoma
Organism Cloning
Chromosomes
Immunoglobulins
Immunoglobulin Heavy Chains
Genetic Translocation
Genomic Library
Metaphase
Southern Blotting
Fluorescence In Situ Hybridization
Gene Library
Northern Blotting
Bacteriophages
Lymphocytes
Biopsy
Cell Line
Messenger RNA
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cloning of bcl-6, the locus involved in chromosome translocations affecting band 3q27 in B-cell lymphoma. / Ye, B. Hilda; Rao, P. H.; Chaganti, R. S K; Dalla-Favera, Riccardo.

In: Cancer Research, Vol. 53, No. 12, 15.06.1993, p. 2732-2735.

Research output: Contribution to journalArticle

Ye, BH, Rao, PH, Chaganti, RSK & Dalla-Favera, R 1993, 'Cloning of bcl-6, the locus involved in chromosome translocations affecting band 3q27 in B-cell lymphoma', Cancer Research, vol. 53, no. 12, pp. 2732-2735.
Ye, B. Hilda ; Rao, P. H. ; Chaganti, R. S K ; Dalla-Favera, Riccardo. / Cloning of bcl-6, the locus involved in chromosome translocations affecting band 3q27 in B-cell lymphoma. In: Cancer Research. 1993 ; Vol. 53, No. 12. pp. 2732-2735.
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