Cloning of a novel nucleolar guanosine 5′-triphosphate binding protein autoantigen from a breast tumor

Janis Racevskis, Alyssa Dill, Richard Stockert, Susan A. Fineberg

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A cDNA clone encoding an immunoreactive autoantigen (Ngp-1) was isolated by screening λgt11 human ductal breast tumor expression libraries with autologous patient serum. The complete 2.3-kb nucleotide sequence of the cDNA was found to contain an open reading frame that could encode a protein of 731 amino acids. The predicted amino acid sequence contains a high concentration of charged amino acids in the carboxy terminal quarter of the molecule, three guanosine 5′-triphosphate (GTP)-binding protein motifs, and a consensus nuclear localization signal. The arrangement and spacing of the GTP-binding protein motifs indicate that Ngp-1 belongs to a newly described subfamily of GTPases. Except for the consensus motifs, neither nucleotide sequence, nor the predicted amino acid sequence of the Ngp-1 cDNA showed the slightest homology to any vertebrate gene product sequence listed in the databases. Northern blot analysis showed the 2.3-kb transcript to be ubiquitously expressed at relatively low levels in all human tissues tested, with the highest level of expression in the testes. Immunohistochemical analysis of tissue sections with affinity-purified antiserum raised against a recombinant Ngp-1 protein revealed that the antigen was exclusively localized to the nucleolus and nucleolar organizer regions in all cell types analyzed.

Original languageEnglish (US)
Pages (from-to)271-280
Number of pages10
JournalCell Growth and Differentiation
Volume7
Issue number2
StatePublished - Feb 1996

Fingerprint

Autoantigens
Guanosine Triphosphate
Nucleolus Organizer Region
Organism Cloning
Carrier Proteins
Amino Acid Motifs
Complementary DNA
Breast Neoplasms
Amino Acid Sequence
Amino Acids
Nuclear Localization Signals
GTP Phosphohydrolases
Northern Blotting
Open Reading Frames
Vertebrates
Testis
Immune Sera
Proteins
Clone Cells
Databases

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Cloning of a novel nucleolar guanosine 5′-triphosphate binding protein autoantigen from a breast tumor. / Racevskis, Janis; Dill, Alyssa; Stockert, Richard; Fineberg, Susan A.

In: Cell Growth and Differentiation, Vol. 7, No. 2, 02.1996, p. 271-280.

Research output: Contribution to journalArticle

Racevskis, Janis ; Dill, Alyssa ; Stockert, Richard ; Fineberg, Susan A. / Cloning of a novel nucleolar guanosine 5′-triphosphate binding protein autoantigen from a breast tumor. In: Cell Growth and Differentiation. 1996 ; Vol. 7, No. 2. pp. 271-280.
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