Cloning, localization, and axonemal function of tetrahymena centrin

Charles Guerra, Yuuko Wada, Vagn Leick, Aaron Bell, Peter Satir

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Centrin, an EF hand Ca2+ binding protein, has been cloned in Tetrahymena thermophila. It is a 167 amino acid protein of 19.4 kDa with a unique N-terminal region, coded by a single gene containing an 85-base pair intron. It has > 80% homology to other centrins and high homology to Tetrahymena EF hand proteins calmodulin, TCBP23, and TCBP25. Specific cellular localizations of the closely related Tetrahymena EF hand proteins are different from centrin. Centrin is localized to basal bodies, cortical fibers in oral apparatus and ciliary rootlets, the apical filament ring and to inner arm (14S) dynein (IAD) along the ciliary axoneme. The function of centrin in Ca2+ control of IAD activity was explored using in vitro microtubule (MT) motility assays. Ca2+ or the Ca2+-mimicking peptide CALP1, which binds EF hand proteins in the absence of Ca2+, increased MT sliding velocity. Antibodies to centrin abrogated this increase. This is the first demonstration of a specific centrin function associated with axonemal dynein. It suggests that centrin is a key regulatory protein for Tetrahymena axonemal Ca2+ responses, including ciliary reversal or chemotaxis.

Original languageEnglish (US)
Pages (from-to)251-261
Number of pages11
JournalMolecular biology of the cell
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Cloning, localization, and axonemal function of tetrahymena centrin'. Together they form a unique fingerprint.

  • Cite this