Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA (hPGT)

Run Lu, Naoaki Kanai, Yi Bao, Victor L. Schuster

Research output: Contribution to journalArticle

183 Scopus citations

Abstract

We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino acid identity compared to rat PGT. When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF(2α) and, to a lesser degree TXB2. Northern blotting revealed mRNA transcripts of many different sizes in adult human heart, placenta, brain, lung, liver, skeletal muscle, pancreas, kidney, spleen, prostate, ovary, small intestine, and colon. hPGT mRNAs are also strongly expressed in human fetal brain, lung, liver, and kidney. The broad tissue distribution and substrate profile of hPGT suggest a role in the transport and/or metabolic clearance of PGs in diverse human tissues.

Original languageEnglish (US)
Pages (from-to)1142-1149
Number of pages8
JournalJournal of Clinical Investigation
Volume98
Issue number5
DOIs
StatePublished - Sep 1 1996

Keywords

  • biological transport
  • carrier proteins
  • molecular cloning
  • prostaglandins

ASJC Scopus subject areas

  • Medicine(all)

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