TY - JOUR
T1 - Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA (hPGT)
AU - Lu, Run
AU - Kanai, Naoaki
AU - Bao, Yi
AU - Schuster, Victor L.
PY - 1996/9/1
Y1 - 1996/9/1
N2 - We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino acid identity compared to rat PGT. When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF(2α) and, to a lesser degree TXB2. Northern blotting revealed mRNA transcripts of many different sizes in adult human heart, placenta, brain, lung, liver, skeletal muscle, pancreas, kidney, spleen, prostate, ovary, small intestine, and colon. hPGT mRNAs are also strongly expressed in human fetal brain, lung, liver, and kidney. The broad tissue distribution and substrate profile of hPGT suggest a role in the transport and/or metabolic clearance of PGs in diverse human tissues.
AB - We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino acid identity compared to rat PGT. When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF(2α) and, to a lesser degree TXB2. Northern blotting revealed mRNA transcripts of many different sizes in adult human heart, placenta, brain, lung, liver, skeletal muscle, pancreas, kidney, spleen, prostate, ovary, small intestine, and colon. hPGT mRNAs are also strongly expressed in human fetal brain, lung, liver, and kidney. The broad tissue distribution and substrate profile of hPGT suggest a role in the transport and/or metabolic clearance of PGs in diverse human tissues.
KW - biological transport
KW - carrier proteins
KW - molecular cloning
KW - prostaglandins
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U2 - 10.1172/JCI118897
DO - 10.1172/JCI118897
M3 - Article
C2 - 8787677
AN - SCOPUS:0029739973
SN - 0021-9738
VL - 98
SP - 1142
EP - 1149
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 5
ER -