TY - JOUR
T1 - Cloning and sequence analysis of the murine glucagon receptor-encoding gene
AU - Burcelin, Rémy
AU - Li, Jing
AU - Charron, Maureen J.
N1 - Funding Information:
We thank Dr. E. Katz, Dr. Jonathan Backer, Dr. S. Efrat, Dr. David Shafritz,A . Stenbit and T.S. Tsao for helpful criticisms. M.J.C. is a recipient of a Career DevelopmentA ward from the American Diabetes Associationa nd is a Scholar of the PEW Charitable Trust. R.B. is the recipient of fellowshipsf rom the Philippe FoundationI,n stitutF ran~aisd e Nutrition and the Associationd e Langue Fran9aisep our rEtude du Diabrte et des Maladies MrtaboliquesT. his work was supportebdy the AmericanD iabetesA ssociationa ndthe DiabetesR esearcha nd Training Center (DK20541-17), the Liver Center( 5P30DK41296a) nd the CancerC enter (5P30CA13330o) f Albert EinsteinC ollegeo f Medicine.
PY - 1995/10/27
Y1 - 1995/10/27
N2 - The glucagon receptor (GR) plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. To study the integrated role of a candidate gene such as GR in wholebody glucose homeostasis by molecular genetic manipulation, cloning of the gene is required. We have cloned and sequenced the murine GR cDNA, gene and promoter region, and studied its tissue distribution. Murine GR contains 13 exons which are located in a region of 4.0 kb. GR encodes a 485-amino-acid protein that consists of seven putative transmembrane domains. By RT-PCR analysis, we have determined that GR is expressed predominantly in liver, kidney, adrenal, lung and stomach, while lower levels of expression are detected in brown and white adipose tissue, cerebellum, duodenum and heart. In addition, a 1000-bp region of the GR promoter has been sequenced which contains consensus sequences for putative DNA-binding proteins involved in tissue specificity (c/EBP; HNFI) or hormonal regulation (steroid receptor). Other consensus sequences known to function in controlling basal promoter activity, such as AP1, AP2 and Spl, are also present. Conversely, no evident TATA or CAAT boxes are present. We provide here important new information necessary for the future pursuit of genetic manipulations in the mouse.
AB - The glucagon receptor (GR) plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. To study the integrated role of a candidate gene such as GR in wholebody glucose homeostasis by molecular genetic manipulation, cloning of the gene is required. We have cloned and sequenced the murine GR cDNA, gene and promoter region, and studied its tissue distribution. Murine GR contains 13 exons which are located in a region of 4.0 kb. GR encodes a 485-amino-acid protein that consists of seven putative transmembrane domains. By RT-PCR analysis, we have determined that GR is expressed predominantly in liver, kidney, adrenal, lung and stomach, while lower levels of expression are detected in brown and white adipose tissue, cerebellum, duodenum and heart. In addition, a 1000-bp region of the GR promoter has been sequenced which contains consensus sequences for putative DNA-binding proteins involved in tissue specificity (c/EBP; HNFI) or hormonal regulation (steroid receptor). Other consensus sequences known to function in controlling basal promoter activity, such as AP1, AP2 and Spl, are also present. Conversely, no evident TATA or CAAT boxes are present. We provide here important new information necessary for the future pursuit of genetic manipulations in the mouse.
KW - G-protein-coupled seven-transmembrane receptor
KW - promoter
UR - http://www.scopus.com/inward/record.url?scp=0028801240&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028801240&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(95)00472-I
DO - 10.1016/0378-1119(95)00472-I
M3 - Article
C2 - 7590348
AN - SCOPUS:0028801240
SN - 0378-1119
VL - 164
SP - 305
EP - 310
JO - Gene
JF - Gene
IS - 2
ER -