Cloning and sequence analysis of cDNA for bovine carboxypeptidase E

Lloyd D. Fricker, Chris J. Evans, Fred S. Esch, Edward Herbert

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Carboxypeptidase E (enkephalin convertase) was first identified as the carboxypeptidase B-like enzyme involved in the biosynthesis of enkephalin in bovine adrenal chromaffin granules1. A similar enzyme is present in many brain regions1,2 and in purified secretory granules from rat pituitary3 and rat insulinoma4. Within the secretory granules, carboxypeptidase E (CPE) activity is found in both a soluble and a membrane-bound form1, which differ slightly in relative molecular mass (Mr)5. Here, to investigate whether the CPE activities in the various tissues are produced from a single gene, purified CPE was partially sequenced and oligonucleotide probes were used to isolate a clone encoding CPE from a bovine pituitary complementary DNA library. This cDNA hybridizes to bovine pituitary poly(A)+ RNAs of approximately 3.3, 2.6 and 2.1 kilobases (kb), with the 3.3-kb messenger RNA the predominant species. The predicted amino-acid sequence of the cDNA clone contains the partially determined sequences of CPE, several pairs of basic amino acids and displays some homology with both carboxypeptidases A and B. Restriction analysis of bovine genomic DNA suggests only one gene for CPE. This is consistent with a broad role for CPE in the biosynthesis of many neuropeptides.

Original languageEnglish (US)
Pages (from-to)461-464
Number of pages4
JournalNature
Volume323
Issue number6087
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Carboxypeptidase H
Sequence Analysis
Organism Cloning
Complementary DNA
Carboxypeptidase B
Secretory Vesicles
Clone Cells
Carboxypeptidases A
Basic Amino Acids
Messenger RNA
Oligonucleotide Probes
Enkephalins
Enzymes
Neuropeptides
Gene Library
Genes
Amino Acid Sequence

ASJC Scopus subject areas

  • General

Cite this

Cloning and sequence analysis of cDNA for bovine carboxypeptidase E. / Fricker, Lloyd D.; Evans, Chris J.; Esch, Fred S.; Herbert, Edward.

In: Nature, Vol. 323, No. 6087, 1986, p. 461-464.

Research output: Contribution to journalArticle

Fricker, LD, Evans, CJ, Esch, FS & Herbert, E 1986, 'Cloning and sequence analysis of cDNA for bovine carboxypeptidase E', Nature, vol. 323, no. 6087, pp. 461-464. https://doi.org/10.1038/323461a0
Fricker, Lloyd D. ; Evans, Chris J. ; Esch, Fred S. ; Herbert, Edward. / Cloning and sequence analysis of cDNA for bovine carboxypeptidase E. In: Nature. 1986 ; Vol. 323, No. 6087. pp. 461-464.
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