Cloning and in situ localization of a brain-derived porin that constitutes a large-conductance anion channel in astrocytic plasma membranes

Rolf Dermietzel, Theng Khing Hwang, Reinhard Buettner, Andreas Hofer, Elisabeth Dotzler, Marian Kremer, Rainer Deutzmann, Friedrich P. Thinnes, Glenn I. Fishman, David C. Spray, Detlef Siemen

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We have cloned a protein from bovine brain, brain-derived voltage- dependent anion channel 1 (BR1-VDAC), that is identical to a recently sequenced plasmalemmal-bound porin from human lymphocytes. mRNA hybridization indicates that BR1-VDAC is widely distributed throughout nervous and nonnervous tissues. In situ localization substantiated that the BR1-VDAC is associated with the plasmalemma of astrocytes. A monoclonal antibody that recognizes the N terminus of the BR1-VDAC protein completely blocks an astrocytic high-conductance anion channel that has electrophysiological similarities with the mitochondrial VDAC. Since the high-conductance anion channel in astrocytes has been shown to respond to hypoosmotic solutions, its molecular identification provides the basis for a better understanding of volume regulation in brain tissue.

Original languageEnglish (US)
Pages (from-to)499-503
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number2
StatePublished - Jan 18 1994


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