Cloning and in situ localization of a brain-derived porin that constitutes a large-conductance anion channel in astrocytic plasma membranes

Rolf Dermietzel; Theng Khing Hwang; Reinhard Buettner; Andreas Hofer; Elisabeth Dotzler; Marian Kremer; Rainer Deutzmann; Friedrich P. Thinnes; Glenn I. Fishman; David C. Spray; Detlef Siemen

doi:10.1073/pnas.91.2.499

Cloning and in situ localization of a brain-derived porin that constitutes a large-conductance anion channel in astrocytic plasma membranes

Rolf Dermietzel, Theng Khing Hwang, Reinhard Buettner, Andreas Hofer, Elisabeth Dotzler, Marian Kremer, Rainer Deutzmann, Friedrich P. Thinnes, Glenn I. Fishman, David C. Spray, Detlef Siemen

Neuroscience

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144 Scopus citations

Abstract

We have cloned a protein from bovine brain, brain-derived voltage- dependent anion channel 1 (BR1-VDAC), that is identical to a recently sequenced plasmalemmal-bound porin from human lymphocytes. mRNA hybridization indicates that BR1-VDAC is widely distributed throughout nervous and nonnervous tissues. In situ localization substantiated that the BR1-VDAC is associated with the plasmalemma of astrocytes. A monoclonal antibody that recognizes the N terminus of the BR1-VDAC protein completely blocks an astrocytic high-conductance anion channel that has electrophysiological similarities with the mitochondrial VDAC. Since the high-conductance anion channel in astrocytes has been shown to respond to hypoosmotic solutions, its molecular identification provides the basis for a better understanding of volume regulation in brain tissue.

Original language	English (US)
Pages (from-to)	499-503
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	2
DOIs	https://doi.org/10.1073/pnas.91.2.499
State	Published - Jan 18 1994

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Dermietzel, R., Hwang, T. K., Buettner, R., Hofer, A., Dotzler, E., Kremer, M., Deutzmann, R., Thinnes, F. P., Fishman, G. I., Spray, D. C., & Siemen, D. (1994). Cloning and in situ localization of a brain-derived porin that constitutes a large-conductance anion channel in astrocytic plasma membranes. Proceedings of the National Academy of Sciences of the United States of America, 91(2), 499-503. https://doi.org/10.1073/pnas.91.2.499

Cloning and in situ localization of a brain-derived porin that constitutes a large-conductance anion channel in astrocytic plasma membranes. / Dermietzel, Rolf; Hwang, Theng Khing; Buettner, Reinhard et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 2, 18.01.1994, p. 499-503.

Research output: Contribution to journal › Article › peer-review

Dermietzel, R, Hwang, TK, Buettner, R, Hofer, A, Dotzler, E, Kremer, M, Deutzmann, R, Thinnes, FP, Fishman, GI, Spray, DC & Siemen, D 1994, 'Cloning and in situ localization of a brain-derived porin that constitutes a large-conductance anion channel in astrocytic plasma membranes', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 2, pp. 499-503. https://doi.org/10.1073/pnas.91.2.499

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abstract = "We have cloned a protein from bovine brain, brain-derived voltage- dependent anion channel 1 (BR1-VDAC), that is identical to a recently sequenced plasmalemmal-bound porin from human lymphocytes. mRNA hybridization indicates that BR1-VDAC is widely distributed throughout nervous and nonnervous tissues. In situ localization substantiated that the BR1-VDAC is associated with the plasmalemma of astrocytes. A monoclonal antibody that recognizes the N terminus of the BR1-VDAC protein completely blocks an astrocytic high-conductance anion channel that has electrophysiological similarities with the mitochondrial VDAC. Since the high-conductance anion channel in astrocytes has been shown to respond to hypoosmotic solutions, its molecular identification provides the basis for a better understanding of volume regulation in brain tissue.",

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AU - Hofer, Andreas

AU - Dotzler, Elisabeth

AU - Kremer, Marian

AU - Deutzmann, Rainer

AU - Thinnes, Friedrich P.

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AU - Spray, David C.

AU - Siemen, Detlef

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Cloning and in situ localization of a brain-derived porin that constitutes a large-conductance anion channel in astrocytic plasma membranes

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