Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR

Jin Zhong Chen, Shu Wang, Rong Tang, Quan Sheng Yang, Enpeng Zhao, Yaoqiong Chao, Kang Ying, Yi Xie, Yu Min Mao

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A cDNA was isolated from the fetal brain cDNA library by high throughput cDNA sequencing. The 2390 bp cDNA with an open reading fragment (ORF) of 816 bp encodes a 272 amino acids putative protein with a thrombospondin type I repeat (TSR) domain and a cysteine-rich region at the N-terminus, so it is named hPWTSR. We used Northern blot detected two bands with length of about 3 kb and 4 kb respectively, which expressed in human adult tissues with different intensities. The expression pattern was verified by RT-PCR, revealing that the transcripts were expressed ubiquitously in fetal tissues and human tumor tissues too. However, the transcript was detected neither in ovarian carcinoma GI-102 nor in lung carcinoma LX-1. Blast analysis against NCBI database revealed that the new gene contained at least 5 exons and located in human chromosome 6q22.33. Our results demonstrate that the gene is a novel member of TSR supergene family.

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalMolecular Biology Reports
Volume29
Issue number3
DOIs
StatePublished - Sep 2002
Externally publishedYes

Fingerprint

Thrombospondins
Cloning
Organism Cloning
Complementary DNA
Tissue
Genes
Carcinoma
Proteins
Human Chromosomes
Chromosomes
Gene Library
Northern Blotting
Cysteine
Tumors
Reading
Exons
Brain
Fetus
Throughput
Databases

Keywords

  • Cysteine-rich region
  • hPWTSR
  • Thrombospondin (TSP)
  • Thrombospondin type I repeat (TSR)

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Biochemistry

Cite this

Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR. / Chen, Jin Zhong; Wang, Shu; Tang, Rong; Yang, Quan Sheng; Zhao, Enpeng; Chao, Yaoqiong; Ying, Kang; Xie, Yi; Mao, Yu Min.

In: Molecular Biology Reports, Vol. 29, No. 3, 09.2002, p. 287-292.

Research output: Contribution to journalArticle

Chen, JZ, Wang, S, Tang, R, Yang, QS, Zhao, E, Chao, Y, Ying, K, Xie, Y & Mao, YM 2002, 'Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR', Molecular Biology Reports, vol. 29, no. 3, pp. 287-292. https://doi.org/10.1023/A:1020479301379
Chen, Jin Zhong ; Wang, Shu ; Tang, Rong ; Yang, Quan Sheng ; Zhao, Enpeng ; Chao, Yaoqiong ; Ying, Kang ; Xie, Yi ; Mao, Yu Min. / Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR. In: Molecular Biology Reports. 2002 ; Vol. 29, No. 3. pp. 287-292.
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