Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR

Jin Zhong Chen, Shu Wang, Rong Tang, Quan Sheng Yang, Enpeng Zhao, Yaoqiong Chao, Kang Ying, Yi Xie, Yu Min Mao

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

A cDNA was isolated from the fetal brain cDNA library by high throughput cDNA sequencing. The 2390 bp cDNA with an open reading fragment (ORF) of 816 bp encodes a 272 amino acids putative protein with a thrombospondin type I repeat (TSR) domain and a cysteine-rich region at the N-terminus, so it is named hPWTSR. We used Northern blot detected two bands with length of about 3 kb and 4 kb respectively, which expressed in human adult tissues with different intensities. The expression pattern was verified by RT-PCR, revealing that the transcripts were expressed ubiquitously in fetal tissues and human tumor tissues too. However, the transcript was detected neither in ovarian carcinoma GI-102 nor in lung carcinoma LX-1. Blast analysis against NCBI database revealed that the new gene contained at least 5 exons and located in human chromosome 6q22.33. Our results demonstrate that the gene is a novel member of TSR supergene family.

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalMolecular Biology Reports
Volume29
Issue number3
DOIs
StatePublished - Sep 1 2002

Keywords

  • Cysteine-rich region
  • Thrombospondin (TSP)
  • Thrombospondin type I repeat (TSR)
  • hPWTSR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Chen, J. Z., Wang, S., Tang, R., Yang, Q. S., Zhao, E., Chao, Y., Ying, K., Xie, Y., & Mao, Y. M. (2002). Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR. Molecular Biology Reports, 29(3), 287-292. https://doi.org/10.1023/A:1020479301379