Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of camp-dependent protein kinase IIβ

Allen H. Hirsch, Susan B. Glantz, Ying Li, Young You, Charles S. Rubin

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The A-Kinase Anchor Protein AKAP 75 (formerly designated bovine brain P75) is a particulate brain protein that avidly binds the regulatory subunit (RIIβ) of cAMP-dependent protein kinase IIβ (Bregman, D. B., Hirsch, A. H. and Rubin, C. S. (1991) J. Biol. Chem. 266, 7207-7213). The formation of stable AKAP 75·RIIβ complexes provides a potential mechanism for targeting physiological signals carried by cAMP to specific effector sites within neurons and other brain cells. We have now cloned and characterized the AKAP 75 gene. Its coding sequence is novel and unexpectedly short (1284 base pairs) and contains no introns. When the AKAP 75 gene was transfected into HEK 293 cells, a new RIIβ-binding protein with an apparent Mr of 75,000 accumulated. A high proportion (∼65%) of the AKAP 75 gene product was excluded from the cytoplasm and was recovered in the 40,000 × g pellet derived from disrupted transfected cells. In contrast, cells transfected with a construct encoding 249 amino acids from the central and C-terminal regions of AKAP 75 produced an RIIβ-binding protein (apparent Mr = 45,000) that was exclusively cytosolic. AKAP 75 is a novel protein composed of only 428 amino acid residues (Mr = 47, 878). A highly acidic C-terminal region mediates the binding of RIIβ (and cAMP-dependent protein kinase IIβ), whereas a positively charged N-terminal segment contains structural features that are essential for the association of AKAP 75 with the cytoskeleton and/or intracellular membranes.

Original languageEnglish (US)
Pages (from-to)2131-2134
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number4
StatePublished - Feb 5 1992

Fingerprint

Cloning
Protein Subunits
Anchors
Introns
Protein Kinases
Organism Cloning
Brain
Genes
Cyclic AMP-Dependent Protein Kinases
A Kinase Anchor Proteins
Carrier Proteins
Amino Acids
Intracellular Membranes
Proteins
HEK293 Cells
Cytoskeleton
Base Pairing
Neurons
Cytoplasm
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of camp-dependent protein kinase IIβ. / Hirsch, Allen H.; Glantz, Susan B.; Li, Ying; You, Young; Rubin, Charles S.

In: Journal of Biological Chemistry, Vol. 267, No. 4, 05.02.1992, p. 2131-2134.

Research output: Contribution to journalArticle

Hirsch, Allen H. ; Glantz, Susan B. ; Li, Ying ; You, Young ; Rubin, Charles S. / Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of camp-dependent protein kinase IIβ. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 4. pp. 2131-2134.
@article{d74f514264da4551a7d402fbad6bad02,
title = "Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of camp-dependent protein kinase IIβ",
abstract = "The A-Kinase Anchor Protein AKAP 75 (formerly designated bovine brain P75) is a particulate brain protein that avidly binds the regulatory subunit (RIIβ) of cAMP-dependent protein kinase IIβ (Bregman, D. B., Hirsch, A. H. and Rubin, C. S. (1991) J. Biol. Chem. 266, 7207-7213). The formation of stable AKAP 75·RIIβ complexes provides a potential mechanism for targeting physiological signals carried by cAMP to specific effector sites within neurons and other brain cells. We have now cloned and characterized the AKAP 75 gene. Its coding sequence is novel and unexpectedly short (1284 base pairs) and contains no introns. When the AKAP 75 gene was transfected into HEK 293 cells, a new RIIβ-binding protein with an apparent Mr of 75,000 accumulated. A high proportion (∼65{\%}) of the AKAP 75 gene product was excluded from the cytoplasm and was recovered in the 40,000 × g pellet derived from disrupted transfected cells. In contrast, cells transfected with a construct encoding 249 amino acids from the central and C-terminal regions of AKAP 75 produced an RIIβ-binding protein (apparent Mr = 45,000) that was exclusively cytosolic. AKAP 75 is a novel protein composed of only 428 amino acid residues (Mr = 47, 878). A highly acidic C-terminal region mediates the binding of RIIβ (and cAMP-dependent protein kinase IIβ), whereas a positively charged N-terminal segment contains structural features that are essential for the association of AKAP 75 with the cytoskeleton and/or intracellular membranes.",
author = "Hirsch, {Allen H.} and Glantz, {Susan B.} and Ying Li and Young You and Rubin, {Charles S.}",
year = "1992",
month = "2",
day = "5",
language = "English (US)",
volume = "267",
pages = "2131--2134",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "4",

}

TY - JOUR

T1 - Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of camp-dependent protein kinase IIβ

AU - Hirsch, Allen H.

AU - Glantz, Susan B.

AU - Li, Ying

AU - You, Young

AU - Rubin, Charles S.

PY - 1992/2/5

Y1 - 1992/2/5

N2 - The A-Kinase Anchor Protein AKAP 75 (formerly designated bovine brain P75) is a particulate brain protein that avidly binds the regulatory subunit (RIIβ) of cAMP-dependent protein kinase IIβ (Bregman, D. B., Hirsch, A. H. and Rubin, C. S. (1991) J. Biol. Chem. 266, 7207-7213). The formation of stable AKAP 75·RIIβ complexes provides a potential mechanism for targeting physiological signals carried by cAMP to specific effector sites within neurons and other brain cells. We have now cloned and characterized the AKAP 75 gene. Its coding sequence is novel and unexpectedly short (1284 base pairs) and contains no introns. When the AKAP 75 gene was transfected into HEK 293 cells, a new RIIβ-binding protein with an apparent Mr of 75,000 accumulated. A high proportion (∼65%) of the AKAP 75 gene product was excluded from the cytoplasm and was recovered in the 40,000 × g pellet derived from disrupted transfected cells. In contrast, cells transfected with a construct encoding 249 amino acids from the central and C-terminal regions of AKAP 75 produced an RIIβ-binding protein (apparent Mr = 45,000) that was exclusively cytosolic. AKAP 75 is a novel protein composed of only 428 amino acid residues (Mr = 47, 878). A highly acidic C-terminal region mediates the binding of RIIβ (and cAMP-dependent protein kinase IIβ), whereas a positively charged N-terminal segment contains structural features that are essential for the association of AKAP 75 with the cytoskeleton and/or intracellular membranes.

AB - The A-Kinase Anchor Protein AKAP 75 (formerly designated bovine brain P75) is a particulate brain protein that avidly binds the regulatory subunit (RIIβ) of cAMP-dependent protein kinase IIβ (Bregman, D. B., Hirsch, A. H. and Rubin, C. S. (1991) J. Biol. Chem. 266, 7207-7213). The formation of stable AKAP 75·RIIβ complexes provides a potential mechanism for targeting physiological signals carried by cAMP to specific effector sites within neurons and other brain cells. We have now cloned and characterized the AKAP 75 gene. Its coding sequence is novel and unexpectedly short (1284 base pairs) and contains no introns. When the AKAP 75 gene was transfected into HEK 293 cells, a new RIIβ-binding protein with an apparent Mr of 75,000 accumulated. A high proportion (∼65%) of the AKAP 75 gene product was excluded from the cytoplasm and was recovered in the 40,000 × g pellet derived from disrupted transfected cells. In contrast, cells transfected with a construct encoding 249 amino acids from the central and C-terminal regions of AKAP 75 produced an RIIβ-binding protein (apparent Mr = 45,000) that was exclusively cytosolic. AKAP 75 is a novel protein composed of only 428 amino acid residues (Mr = 47, 878). A highly acidic C-terminal region mediates the binding of RIIβ (and cAMP-dependent protein kinase IIβ), whereas a positively charged N-terminal segment contains structural features that are essential for the association of AKAP 75 with the cytoskeleton and/or intracellular membranes.

UR - http://www.scopus.com/inward/record.url?scp=0026787019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026787019&partnerID=8YFLogxK

M3 - Article

VL - 267

SP - 2131

EP - 2134

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 4

ER -