Cloning and characterization of the human gene for the α-subunit of Gi2, a GTP-binding signal transduction protein

L. S. Weinstein, Allen M. Spiegel, A. D. Carter

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We isolated and characterized human genomic clones encompassing the gene for the α-subunit of Gi2, a GTP-binding signal transduction protein abundantly expressed in myeloid cells. The gene is divided into 9 exons and spans 23.5 kb. Exons 2, 6 and 7 encode putative guanine nucleotide-binding domains that are highly conserved among GTP-binding proteins. A polyadenylation signal located within exon 9 predicts an MRNA size (∼ 2.3 kb) several hundred bases longer than that of published cDNAs, and consistent with the size seen on RNA blot hybridization. Primer extension and S1 nuclease analysis determined a major and several minor transcriptional start sites. The first exon and 5′ flanking region are highly G+ C rich, contain several GC boxes (SP1 transcription factor binding sites), a CAAT box, and lack a TATA box. The presumptive promoter region is thus similar to that of ras and other widely expressed genes.

Original languageEnglish (US)
Pages (from-to)333-340
Number of pages8
JournalFEBS Letters
Volume232
Issue number2
DOIs
StatePublished - May 23 1988
Externally publishedYes

Fingerprint

Signal transduction
Cloning
Guanosine Triphosphate
Organism Cloning
Exons
Signal Transduction
Genes
Proteins
TATA Box
Polyadenylation
Guanine Nucleotides
5' Flanking Region
Myeloid Cells
GTP-Binding Proteins
Genetic Promoter Regions
Transcription Factors
Complementary DNA
Clone Cells
Binding Sites
RNA

Keywords

  • G-protein
  • GC box
  • Genomic clone
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning and characterization of the human gene for the α-subunit of Gi2, a GTP-binding signal transduction protein. / Weinstein, L. S.; Spiegel, Allen M.; Carter, A. D.

In: FEBS Letters, Vol. 232, No. 2, 23.05.1988, p. 333-340.

Research output: Contribution to journalArticle

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