Abstract
We isolated and characterized human genomic clones encompassing the gene for the α-subunit of Gi2, a GTP-binding signal transduction protein abundantly expressed in myeloid cells. The gene is divided into 9 exons and spans 23.5 kb. Exons 2, 6 and 7 encode putative guanine nucleotide-binding domains that are highly conserved among GTP-binding proteins. A polyadenylation signal located within exon 9 predicts an MRNA size (∼ 2.3 kb) several hundred bases longer than that of published cDNAs, and consistent with the size seen on RNA blot hybridization. Primer extension and S1 nuclease analysis determined a major and several minor transcriptional start sites. The first exon and 5′ flanking region are highly G+ C rich, contain several GC boxes (SP1 transcription factor binding sites), a CAAT box, and lack a TATA box. The presumptive promoter region is thus similar to that of ras and other widely expressed genes.
Original language | English (US) |
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Pages (from-to) | 333-340 |
Number of pages | 8 |
Journal | FEBS Letters |
Volume | 232 |
Issue number | 2 |
DOIs | |
State | Published - May 23 1988 |
Externally published | Yes |
Keywords
- G-protein
- GC box
- Genomic clone
- Signal transduction
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology