Cloning and characterization of human synaptotagmin 10 gene

Enpeng Zhao, Yao Li, Xuping Fu, Li Zeng, Huawu Zeng, Wei Jin, Jinzhong Chen, Gang Yin, Ji Qian, Kang Ying, Yi Xie, Robert Chunhua Zhao, Yumin Mao

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.

Original languageEnglish (US)
Pages (from-to)393-398
Number of pages6
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume14
Issue number5
DOIs
Publication statusPublished - Oct 1 2003

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Keywords

  • C2 domains
  • Ca
  • Cloning
  • Synaptotagmin 10

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

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