Cloning and characterization of human synaptotagmin 10 gene

Enpeng Zhao, Yao Li, Xuping Fu, Li Zeng, Huawu Zeng, Wei Jin, Jinzhong Chen, Gang Yin, Ji Qian, Kang Ying, Yi Xie, Robert Chunhua Zhao, Yumin Mao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.

Original languageEnglish (US)
Pages (from-to)393-398
Number of pages6
JournalDNA Sequence - Journal of DNA Sequencing and Mapping
Volume14
Issue number5
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Synaptotagmins
Cloning
Organism Cloning
Genes
Gene Library
Rats
Brain
Screening
Membrane Proteins
Proteins
Endocrine Cells
Complementary DNA
Cells
Secretory Vesicles
Amino Acids
Open Reading Frames
Pancreas
Clone Cells
Kidney
Polymerase Chain Reaction

Keywords

  • C2 domains
  • Ca
  • Cloning
  • Synaptotagmin 10

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Cloning and characterization of human synaptotagmin 10 gene. / Zhao, Enpeng; Li, Yao; Fu, Xuping; Zeng, Li; Zeng, Huawu; Jin, Wei; Chen, Jinzhong; Yin, Gang; Qian, Ji; Ying, Kang; Xie, Yi; Zhao, Robert Chunhua; Mao, Yumin.

In: DNA Sequence - Journal of DNA Sequencing and Mapping, Vol. 14, No. 5, 10.2003, p. 393-398.

Research output: Contribution to journalArticle

Zhao, E, Li, Y, Fu, X, Zeng, L, Zeng, H, Jin, W, Chen, J, Yin, G, Qian, J, Ying, K, Xie, Y, Zhao, RC & Mao, Y 2003, 'Cloning and characterization of human synaptotagmin 10 gene', DNA Sequence - Journal of DNA Sequencing and Mapping, vol. 14, no. 5, pp. 393-398. https://doi.org/10.1080/10425170310001608407
Zhao, Enpeng ; Li, Yao ; Fu, Xuping ; Zeng, Li ; Zeng, Huawu ; Jin, Wei ; Chen, Jinzhong ; Yin, Gang ; Qian, Ji ; Ying, Kang ; Xie, Yi ; Zhao, Robert Chunhua ; Mao, Yumin. / Cloning and characterization of human synaptotagmin 10 gene. In: DNA Sequence - Journal of DNA Sequencing and Mapping. 2003 ; Vol. 14, No. 5. pp. 393-398.
@article{70c7a90af11e4d2d804cbb904eb13932,
title = "Cloning and characterization of human synaptotagmin 10 gene",
abstract = "Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8{\%} homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.",
keywords = "C2 domains, Ca, Cloning, Synaptotagmin 10",
author = "Enpeng Zhao and Yao Li and Xuping Fu and Li Zeng and Huawu Zeng and Wei Jin and Jinzhong Chen and Gang Yin and Ji Qian and Kang Ying and Yi Xie and Zhao, {Robert Chunhua} and Yumin Mao",
year = "2003",
month = "10",
doi = "10.1080/10425170310001608407",
language = "English (US)",
volume = "14",
pages = "393--398",
journal = "DNA Sequence - Journal of DNA Sequencing and Mapping",
issn = "1940-1736",
publisher = "Informa Healthcare",
number = "5",

}

TY - JOUR

T1 - Cloning and characterization of human synaptotagmin 10 gene

AU - Zhao, Enpeng

AU - Li, Yao

AU - Fu, Xuping

AU - Zeng, Li

AU - Zeng, Huawu

AU - Jin, Wei

AU - Chen, Jinzhong

AU - Yin, Gang

AU - Qian, Ji

AU - Ying, Kang

AU - Xie, Yi

AU - Zhao, Robert Chunhua

AU - Mao, Yumin

PY - 2003/10

Y1 - 2003/10

N2 - Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.

AB - Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.

KW - C2 domains

KW - Ca

KW - Cloning

KW - Synaptotagmin 10

UR - http://www.scopus.com/inward/record.url?scp=0141642047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141642047&partnerID=8YFLogxK

U2 - 10.1080/10425170310001608407

DO - 10.1080/10425170310001608407

M3 - Article

VL - 14

SP - 393

EP - 398

JO - DNA Sequence - Journal of DNA Sequencing and Mapping

JF - DNA Sequence - Journal of DNA Sequencing and Mapping

SN - 1940-1736

IS - 5

ER -