Cloning and characterization of human synaptotagmin 10 gene

Enpeng Zhao; Yao Li; Xuping Fu; Li Zeng; Huawu Zeng; Wei Jin; Jinzhong Chen; Gang Yin; Ji Qian; Kang Ying; Yi Xie; Robert Chunhua Zhao; Yumin Mao

doi:10.1080/10425170310001608407

Cloning and characterization of human synaptotagmin 10 gene

Enpeng Zhao, Yao Li, Xuping Fu, Li Zeng, Huawu Zeng, Wei Jin, Jinzhong Chen, Gang Yin, Ji Qian, Kang Ying, Yi Xie, Robert Chunhua Zhao, Yumin Mao

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.

Original language	English (US)
Pages (from-to)	393-398
Number of pages	6
Journal	DNA Sequence - Journal of DNA Sequencing and Mapping
Volume	14
Issue number	5
DOIs	https://doi.org/10.1080/10425170310001608407
State	Published - Oct 2003
Externally published	Yes

Keywords

C2 domains
Ca
Cloning
Synaptotagmin 10

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics
Endocrinology

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10.1080/10425170310001608407

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title = "Cloning and characterization of human synaptotagmin 10 gene",

abstract = "Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.",

keywords = "C2 domains, Ca, Cloning, Synaptotagmin 10",

author = "Enpeng Zhao and Yao Li and Xuping Fu and Li Zeng and Huawu Zeng and Wei Jin and Jinzhong Chen and Gang Yin and Ji Qian and Kang Ying and Yi Xie and Zhao, {Robert Chunhua} and Yumin Mao",

note = "Funding Information: We gratefully acknowledge financial support from the 863 project of People{\textquoteright}s Republic of China (grant number 2001AA221181) and National Natural Science Foundation of China (grant number 30170315).",

year = "2003",

month = oct,

doi = "10.1080/10425170310001608407",

language = "English (US)",

volume = "14",

pages = "393--398",

journal = "DNA Sequence - Journal of DNA Sequencing and Mapping",

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TY - JOUR

T1 - Cloning and characterization of human synaptotagmin 10 gene

AU - Zhao, Enpeng

AU - Li, Yao

AU - Fu, Xuping

AU - Zeng, Li

AU - Zeng, Huawu

AU - Jin, Wei

AU - Chen, Jinzhong

AU - Yin, Gang

AU - Qian, Ji

AU - Ying, Kang

AU - Xie, Yi

AU - Zhao, Robert Chunhua

AU - Mao, Yumin

N1 - Funding Information: We gratefully acknowledge financial support from the 863 project of People’s Republic of China (grant number 2001AA221181) and National Natural Science Foundation of China (grant number 30170315).

PY - 2003/10

Y1 - 2003/10

N2 - Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.

AB - Synaptotagmin (Syt) is a membrane protein family of secretory vesicles, abundant in neural and some endocrine cells. All the members of this family contain one transmembrane region and two conserved C2 domains. Here we reported a new human Syt 10 gene isolated when screening a human fetal brain cDNA library. This cDNA clone is 3287 bp and contains an open reading frame from 299 to 1870 encoding a putative protein of 523 amino acids. It shares 94.6 and 94. 8% homology to rat Syt 10 and mouse Syt 10 at protein level, respectively. RT-PCR result showed that it is expressed only in pancreas, lung and kidney.

KW - C2 domains

KW - Ca

KW - Cloning

KW - Synaptotagmin 10

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SP - 393

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JO - DNA Sequence - Journal of DNA Sequencing and Mapping

JF - DNA Sequence - Journal of DNA Sequencing and Mapping

IS - 5

ER -

Cloning and characterization of human synaptotagmin 10 gene

Abstract

Keywords

ASJC Scopus subject areas

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