Clinical, Histological, and Molecular Markers Associated With Allograft Loss in Transplant Glomerulopathy Patients

Layla Kamal, Pilib Broin, Yi Bao, Maria Ajaimy, Michelle Lubetzky, Anjali Gupta, Graciela de Boccardo, James M. Pullman, Aaron Golden, Enver Akalin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

RESULTS: Over a median follow up of 23 months (1-46 months) after the diagnosis of TGP by biopsy, 17 patients (32%) lost their allografts at a median of 16 months (1-44 months). There was no difference between the 2 groups in terms of any demographic variables, serum creatinine, panel reactive antibody levels, donor-specific antibody frequency, or mean fluorescence intensity values. Patients who lost their allograft had a significantly higher median spot protein to creatinine ratio 2.81 (1.20-6.00) compared to no graft loss patients 1.16 (0.15-2.53), (P <0.01), and a trends toward a higher mean chronic glomerulopathy (cg) score (1.65 ± 0.93 vs 1.11 ± 0.93) (P = 0.05). There was also no difference in microvascular inflammation or any other Banff injury scores between the 2 groups. Although 117 gene transcripts were upregulated in both groups, 86 and 57 were upregulated in graft loss and functioning allograft groups, respectively. There were significantly increased levels of intragraft endothelial cell-associated transcripts, gene transcripts associated with complement cascade, interleukins and their receptors and granulysin in graft loss patients compared to patients with a functioning allograft.

CONCLUSION: Our results demonstrate differential intragraft gene expression profiles in TGP patients with allograft loss.

BACKGROUND: We aimed to investigate the clinical, histopathological, and molecular factors associated with allograft loss in transplant glomerulopathy (TGP) patients.

METHODS: Of the 525 patients who underwent clinically indicated kidney biopsies, 52 (10%) had diagnosis of TGP. Gene expression profiles of 28 TGP and 11 normal transplant kidney biopsy samples were analyzed by Affymetrix HuGene 1.0 ST expression arrays.

Original languageEnglish (US)
Pages (from-to)1912-1918
Number of pages7
JournalTransplantation
Volume99
Issue number9
DOIs
StatePublished - Sep 1 2015

Fingerprint

Allografts
Transplants
Biopsy
Transcriptome
Creatinine
Interleukin Receptors
Kidney
Antibodies
Genes
Endothelial Cells
Fluorescence
Demography
Tissue Donors
Inflammation
Wounds and Injuries
Serum
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Clinical, Histological, and Molecular Markers Associated With Allograft Loss in Transplant Glomerulopathy Patients. / Kamal, Layla; Broin, Pilib; Bao, Yi; Ajaimy, Maria; Lubetzky, Michelle; Gupta, Anjali; de Boccardo, Graciela; Pullman, James M.; Golden, Aaron; Akalin, Enver.

In: Transplantation, Vol. 99, No. 9, 01.09.2015, p. 1912-1918.

Research output: Contribution to journalArticle

Kamal, Layla ; Broin, Pilib ; Bao, Yi ; Ajaimy, Maria ; Lubetzky, Michelle ; Gupta, Anjali ; de Boccardo, Graciela ; Pullman, James M. ; Golden, Aaron ; Akalin, Enver. / Clinical, Histological, and Molecular Markers Associated With Allograft Loss in Transplant Glomerulopathy Patients. In: Transplantation. 2015 ; Vol. 99, No. 9. pp. 1912-1918.
@article{6ef1480a16dc4e779a31d03beb003067,
title = "Clinical, Histological, and Molecular Markers Associated With Allograft Loss in Transplant Glomerulopathy Patients",
abstract = "RESULTS: Over a median follow up of 23 months (1-46 months) after the diagnosis of TGP by biopsy, 17 patients (32{\%}) lost their allografts at a median of 16 months (1-44 months). There was no difference between the 2 groups in terms of any demographic variables, serum creatinine, panel reactive antibody levels, donor-specific antibody frequency, or mean fluorescence intensity values. Patients who lost their allograft had a significantly higher median spot protein to creatinine ratio 2.81 (1.20-6.00) compared to no graft loss patients 1.16 (0.15-2.53), (P <0.01), and a trends toward a higher mean chronic glomerulopathy (cg) score (1.65 ± 0.93 vs 1.11 ± 0.93) (P = 0.05). There was also no difference in microvascular inflammation or any other Banff injury scores between the 2 groups. Although 117 gene transcripts were upregulated in both groups, 86 and 57 were upregulated in graft loss and functioning allograft groups, respectively. There were significantly increased levels of intragraft endothelial cell-associated transcripts, gene transcripts associated with complement cascade, interleukins and their receptors and granulysin in graft loss patients compared to patients with a functioning allograft.CONCLUSION: Our results demonstrate differential intragraft gene expression profiles in TGP patients with allograft loss.BACKGROUND: We aimed to investigate the clinical, histopathological, and molecular factors associated with allograft loss in transplant glomerulopathy (TGP) patients.METHODS: Of the 525 patients who underwent clinically indicated kidney biopsies, 52 (10{\%}) had diagnosis of TGP. Gene expression profiles of 28 TGP and 11 normal transplant kidney biopsy samples were analyzed by Affymetrix HuGene 1.0 ST expression arrays.",
author = "Layla Kamal and Pilib Broin and Yi Bao and Maria Ajaimy and Michelle Lubetzky and Anjali Gupta and {de Boccardo}, Graciela and Pullman, {James M.} and Aaron Golden and Enver Akalin",
year = "2015",
month = "9",
day = "1",
doi = "10.1097/TP.0000000000000598",
language = "English (US)",
volume = "99",
pages = "1912--1918",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - Clinical, Histological, and Molecular Markers Associated With Allograft Loss in Transplant Glomerulopathy Patients

AU - Kamal, Layla

AU - Broin, Pilib

AU - Bao, Yi

AU - Ajaimy, Maria

AU - Lubetzky, Michelle

AU - Gupta, Anjali

AU - de Boccardo, Graciela

AU - Pullman, James M.

AU - Golden, Aaron

AU - Akalin, Enver

PY - 2015/9/1

Y1 - 2015/9/1

N2 - RESULTS: Over a median follow up of 23 months (1-46 months) after the diagnosis of TGP by biopsy, 17 patients (32%) lost their allografts at a median of 16 months (1-44 months). There was no difference between the 2 groups in terms of any demographic variables, serum creatinine, panel reactive antibody levels, donor-specific antibody frequency, or mean fluorescence intensity values. Patients who lost their allograft had a significantly higher median spot protein to creatinine ratio 2.81 (1.20-6.00) compared to no graft loss patients 1.16 (0.15-2.53), (P <0.01), and a trends toward a higher mean chronic glomerulopathy (cg) score (1.65 ± 0.93 vs 1.11 ± 0.93) (P = 0.05). There was also no difference in microvascular inflammation or any other Banff injury scores between the 2 groups. Although 117 gene transcripts were upregulated in both groups, 86 and 57 were upregulated in graft loss and functioning allograft groups, respectively. There were significantly increased levels of intragraft endothelial cell-associated transcripts, gene transcripts associated with complement cascade, interleukins and their receptors and granulysin in graft loss patients compared to patients with a functioning allograft.CONCLUSION: Our results demonstrate differential intragraft gene expression profiles in TGP patients with allograft loss.BACKGROUND: We aimed to investigate the clinical, histopathological, and molecular factors associated with allograft loss in transplant glomerulopathy (TGP) patients.METHODS: Of the 525 patients who underwent clinically indicated kidney biopsies, 52 (10%) had diagnosis of TGP. Gene expression profiles of 28 TGP and 11 normal transplant kidney biopsy samples were analyzed by Affymetrix HuGene 1.0 ST expression arrays.

AB - RESULTS: Over a median follow up of 23 months (1-46 months) after the diagnosis of TGP by biopsy, 17 patients (32%) lost their allografts at a median of 16 months (1-44 months). There was no difference between the 2 groups in terms of any demographic variables, serum creatinine, panel reactive antibody levels, donor-specific antibody frequency, or mean fluorescence intensity values. Patients who lost their allograft had a significantly higher median spot protein to creatinine ratio 2.81 (1.20-6.00) compared to no graft loss patients 1.16 (0.15-2.53), (P <0.01), and a trends toward a higher mean chronic glomerulopathy (cg) score (1.65 ± 0.93 vs 1.11 ± 0.93) (P = 0.05). There was also no difference in microvascular inflammation or any other Banff injury scores between the 2 groups. Although 117 gene transcripts were upregulated in both groups, 86 and 57 were upregulated in graft loss and functioning allograft groups, respectively. There were significantly increased levels of intragraft endothelial cell-associated transcripts, gene transcripts associated with complement cascade, interleukins and their receptors and granulysin in graft loss patients compared to patients with a functioning allograft.CONCLUSION: Our results demonstrate differential intragraft gene expression profiles in TGP patients with allograft loss.BACKGROUND: We aimed to investigate the clinical, histopathological, and molecular factors associated with allograft loss in transplant glomerulopathy (TGP) patients.METHODS: Of the 525 patients who underwent clinically indicated kidney biopsies, 52 (10%) had diagnosis of TGP. Gene expression profiles of 28 TGP and 11 normal transplant kidney biopsy samples were analyzed by Affymetrix HuGene 1.0 ST expression arrays.

UR - http://www.scopus.com/inward/record.url?scp=84944108630&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84944108630&partnerID=8YFLogxK

U2 - 10.1097/TP.0000000000000598

DO - 10.1097/TP.0000000000000598

M3 - Article

C2 - 25675205

AN - SCOPUS:84944108630

VL - 99

SP - 1912

EP - 1918

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 9

ER -