The effect of chronic cold stress on the composition and function of rat intestinal brush border membrane (BBM) was studied. Various lipid fractions from intestinal BBM viz. cholesterol (p<0.01), phospholipids (p<0.01), triglycerides (p<0.05) and gangliosides (p<0.05) were significantly reduced in cold stressed animals, as compared to controls. Analysis of membrane saccharide content revealed a significant increase in sialic acid (25%) and hexosamine (36%) contents and a reduction in fucose (19%) content in cold stressed rats. Determination of various enzyme activities in BBM showed significantly enhanced activities of alkaline phosphatase (p<0.01), lactase (p<0.001) and leucine aminopeptidase (p<0.001), whereas sucrase activity was reduced (p<0.05) under these conditions. The magnitude and site of these alterations across the crypt-villus axis varied from enzyme to enzyme. These findings suggest that chronic cold stress results in profound alterations in intestinal BBM. Altered structure and function of intestinal BBM may play a role in stress-induced derangements in gastrointestinal tract.
|Original language||English (US)|
|Number of pages||6|
|Journal||Indian Journal of Biochemistry and Biophysics|
|State||Published - Jun 1 2003|
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