Chromosome Localization of Human Genes for Clathrin Adaptor Polypeptides AP2β and AP50 and the Clathrin-Binding Protein, VCP

TERESA DRUCK, YANSONG GU, GANESH PRABHALA, LINDA A. CANNIZZARO, SUN HWA PARK, KAY HUEBNER, JAMES H. KEEN

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Clathrin-coated vesicles, involved in endocytosis and Golgi processing, have a surface lattice containing clathrin triskelia and stoichiometric amounts of additional components termed "assembly proteins," or APs. The AP form at the plasma membrane, AP2, is composed of two large subunits of 100-115 kDa, denoted AP2α and AP2β, a medium chain of 50 kDa, designated AP50, and a small chain. We have determined human chromosomal locations of genes for a large AP2β (CLAPB1) and a medium (CLAPM1) AP subunit and of a novel clathrin-binding protein, VCP, that binds clathrin simultaneously with APs. Chromosomalin situhybridization of a human genomic clone demonstrated that the CLAPM1 gene mapped to chromosome region 3q28. The gene for the CLAPB1 large subunit was mapped to 17q11.2-q12 by PCR amplification of an AP2β fragment from a panel of rodent-human hybrid DNAs. To map the human VCP sequence, a human-specific probe was made by RT-PCR of human mRNA using oligonucleotide primers from conserved regions of the porcine sequence. The amplified human fragment served as probe on Southern blots of hybrid DNAs to determine that the human VCP locus maps to chromosome region 9pter-q34.

Original languageEnglish (US)
Pages (from-to)94-95
Number of pages2
JournalGenomics
Volume30
Issue number1
DOIs
StatePublished - Nov 1995
Externally publishedYes

Fingerprint

Vesicular Transport Adaptor Proteins
Clathrin
Human Chromosomes
Carrier Proteins
Peptides
Genes
Chromosomes
Clathrin-Coated Vesicles
Polymerase Chain Reaction
DNA Primers
DNA
Southern Blotting
Endocytosis
Rodentia
Swine
Clone Cells
Cell Membrane
Messenger RNA

ASJC Scopus subject areas

  • Genetics

Cite this

DRUCK, TERESA., GU, YANSONG., PRABHALA, GANESH., CANNIZZARO, LINDA. A., PARK, SUN. HWA., HUEBNER, KAY., & KEEN, JAMES. H. (1995). Chromosome Localization of Human Genes for Clathrin Adaptor Polypeptides AP2β and AP50 and the Clathrin-Binding Protein, VCP. Genomics, 30(1), 94-95. https://doi.org/10.1006/geno.1995.0016

Chromosome Localization of Human Genes for Clathrin Adaptor Polypeptides AP2β and AP50 and the Clathrin-Binding Protein, VCP. / DRUCK, TERESA; GU, YANSONG; PRABHALA, GANESH; CANNIZZARO, LINDA A.; PARK, SUN HWA; HUEBNER, KAY; KEEN, JAMES H.

In: Genomics, Vol. 30, No. 1, 11.1995, p. 94-95.

Research output: Contribution to journalArticle

DRUCK, TERESA, GU, YANSONG, PRABHALA, GANESH, CANNIZZARO, LINDAA, PARK, SUNHWA, HUEBNER, KAY & KEEN, JAMESH 1995, 'Chromosome Localization of Human Genes for Clathrin Adaptor Polypeptides AP2β and AP50 and the Clathrin-Binding Protein, VCP', Genomics, vol. 30, no. 1, pp. 94-95. https://doi.org/10.1006/geno.1995.0016
DRUCK TERESA, GU YANSONG, PRABHALA GANESH, CANNIZZARO LINDAA, PARK SUNHWA, HUEBNER KAY et al. Chromosome Localization of Human Genes for Clathrin Adaptor Polypeptides AP2β and AP50 and the Clathrin-Binding Protein, VCP. Genomics. 1995 Nov;30(1):94-95. https://doi.org/10.1006/geno.1995.0016
DRUCK, TERESA ; GU, YANSONG ; PRABHALA, GANESH ; CANNIZZARO, LINDA A. ; PARK, SUN HWA ; HUEBNER, KAY ; KEEN, JAMES H. / Chromosome Localization of Human Genes for Clathrin Adaptor Polypeptides AP2β and AP50 and the Clathrin-Binding Protein, VCP. In: Genomics. 1995 ; Vol. 30, No. 1. pp. 94-95.
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