Chromosome alterations in 21 non-small cell lung carcinomas

I. Miura, J. M. Siegfried, J. Resau, S. M. Keller, J. Y. Zhou, J. R. Testa

Research output: Contribution to journalArticle

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Abstract

Cytogenetic analysis was performed on 16 primary tumors, 2 effusions, and 3 cell lines from 21 patients with non-small cell lung cancer (NSCLC). In 20 patients specimens were obtained prior to initiating cytotoxic therapy. Extensive clonal chromosome alterations were found in all cases. The most frequent numerical changes were polysomy 7 and polysomy 20 (each seen in 12 specimens). In addition, tumor cells from another six cases exhibited partial trisomy 7, with the shortest region of overlap (SRO) at 7p11-p13. Rearrangements of chromosomes 1, 3, 6, 8, 11, 15, 17, and 19 were each observed in nine or more tumors. Breakpoints were clustered at several chromosomal sites, including 1p13, 3p13, 15p11-q11, 17p11, and 19q13. Recurrent loss involving 1p, 3p, 6q, 11p, 15p, 17p, and 19q were each seen in at least eight cases. The SRO of 3p losses was at band 3p21. Double minute chromosomes were found in three tumors. Overall, our findings indicate that even though karyotypes in newly diagnosed NSCLC are very complex, recurrent cytogenetic changes can be identified. The high incidence of loss of 17p (14 of 21 specimens) appears to be compatible with reports implicating the TP53 gene (at band 17p13) as a frequent site for genetic alteration in lung cancer. Moreover, the recurrence of loss of 3p (12 cases) and 11p (10 cases) is also consistent with recent molecular evidence. The existence of other ''hot spots'' for cytogenetic change, particularly those involving specific regions on chromosomes 7, 15, and 19, warrants further molecular investigation of these sites in NSCLC.

Original languageEnglish (US)
Pages (from-to)328-338
Number of pages11
JournalGenes Chromosomes and Cancer
Volume2
Issue number4
StatePublished - 1990
Externally publishedYes

Fingerprint

Non-Small Cell Lung Carcinoma
Chromosomes
Cytogenetics
Neoplasms
Chromosomes, Human, Pair 19
Chromosomes, Human, Pair 15
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 7
Chromosomes, Human, Pair 1
Cytogenetic Analysis
Trisomy
p53 Genes
Karyotype
Lung Neoplasms
Recurrence
Cell Line
Incidence
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Miura, I., Siegfried, J. M., Resau, J., Keller, S. M., Zhou, J. Y., & Testa, J. R. (1990). Chromosome alterations in 21 non-small cell lung carcinomas. Genes Chromosomes and Cancer, 2(4), 328-338.

Chromosome alterations in 21 non-small cell lung carcinomas. / Miura, I.; Siegfried, J. M.; Resau, J.; Keller, S. M.; Zhou, J. Y.; Testa, J. R.

In: Genes Chromosomes and Cancer, Vol. 2, No. 4, 1990, p. 328-338.

Research output: Contribution to journalArticle

Miura, I, Siegfried, JM, Resau, J, Keller, SM, Zhou, JY & Testa, JR 1990, 'Chromosome alterations in 21 non-small cell lung carcinomas', Genes Chromosomes and Cancer, vol. 2, no. 4, pp. 328-338.
Miura I, Siegfried JM, Resau J, Keller SM, Zhou JY, Testa JR. Chromosome alterations in 21 non-small cell lung carcinomas. Genes Chromosomes and Cancer. 1990;2(4):328-338.
Miura, I. ; Siegfried, J. M. ; Resau, J. ; Keller, S. M. ; Zhou, J. Y. ; Testa, J. R. / Chromosome alterations in 21 non-small cell lung carcinomas. In: Genes Chromosomes and Cancer. 1990 ; Vol. 2, No. 4. pp. 328-338.
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