Chromosomal translocation in T-cell leukemia line HUT 78 results in a MYC fusion transcript

L. R. Finger, K. Huebner, L. A. Cannizzaro, K. McLeod, P. C. Nowell, C. M. Croce

Research output: Contribution to journalArticle

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Abstract

Primary cultures and established cell lines derived from human T-cell leukemias were analyzed for genomic rearrangements in the region 3' of the MYC locus. A T-cell leukemia line, HUT 78, whose 3' MYC region is rearranged, carries a chromosome t(2;8) juxtaposition; i.e., a locus derived from chromosome region 2q34 is attached to the 3' end of one MYC allele. The t(2;8) rearrangement in the HUT 78 cell line results in expression of a fused transcript encompassing the MYC gene and a locus designated TCL4 (T-cell leukemia/lymphoma 4), which normally resides on chromosome 2. The steady-state level of MYC-TCL4 fusion transcripts in HUT 78 cells is significantly higher than the MYC RNA level found in several other B- and T-cell lines. The production of fused MYC-TCL4 transcripts in a leukemic cell line raises the possibility that other B- and T-cell leukemias may express MYC fusion transcripts as an integral step in their pathogenesis.

Original languageEnglish (US)
Pages (from-to)9158-9162
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number23
StatePublished - 1988
Externally publishedYes

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T-Cell Leukemia
Genetic Translocation
Cell Line
T-Cell Lymphoma
Chromosomes, Human, Pair 2
B-Cell Leukemia
Chromosomes
Alleles
RNA
T-Lymphocytes
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Finger, L. R., Huebner, K., Cannizzaro, L. A., McLeod, K., Nowell, P. C., & Croce, C. M. (1988). Chromosomal translocation in T-cell leukemia line HUT 78 results in a MYC fusion transcript. Proceedings of the National Academy of Sciences of the United States of America, 85(23), 9158-9162.

Chromosomal translocation in T-cell leukemia line HUT 78 results in a MYC fusion transcript. / Finger, L. R.; Huebner, K.; Cannizzaro, L. A.; McLeod, K.; Nowell, P. C.; Croce, C. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 23, 1988, p. 9158-9162.

Research output: Contribution to journalArticle

Finger, LR, Huebner, K, Cannizzaro, LA, McLeod, K, Nowell, PC & Croce, CM 1988, 'Chromosomal translocation in T-cell leukemia line HUT 78 results in a MYC fusion transcript', Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 23, pp. 9158-9162.
Finger, L. R. ; Huebner, K. ; Cannizzaro, L. A. ; McLeod, K. ; Nowell, P. C. ; Croce, C. M. / Chromosomal translocation in T-cell leukemia line HUT 78 results in a MYC fusion transcript. In: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Vol. 85, No. 23. pp. 9158-9162.
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abstract = "Primary cultures and established cell lines derived from human T-cell leukemias were analyzed for genomic rearrangements in the region 3' of the MYC locus. A T-cell leukemia line, HUT 78, whose 3' MYC region is rearranged, carries a chromosome t(2;8) juxtaposition; i.e., a locus derived from chromosome region 2q34 is attached to the 3' end of one MYC allele. The t(2;8) rearrangement in the HUT 78 cell line results in expression of a fused transcript encompassing the MYC gene and a locus designated TCL4 (T-cell leukemia/lymphoma 4), which normally resides on chromosome 2. The steady-state level of MYC-TCL4 fusion transcripts in HUT 78 cells is significantly higher than the MYC RNA level found in several other B- and T-cell lines. The production of fused MYC-TCL4 transcripts in a leukemic cell line raises the possibility that other B- and T-cell leukemias may express MYC fusion transcripts as an integral step in their pathogenesis.",
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AU - Finger, L. R.

AU - Huebner, K.

AU - Cannizzaro, L. A.

AU - McLeod, K.

AU - Nowell, P. C.

AU - Croce, C. M.

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N2 - Primary cultures and established cell lines derived from human T-cell leukemias were analyzed for genomic rearrangements in the region 3' of the MYC locus. A T-cell leukemia line, HUT 78, whose 3' MYC region is rearranged, carries a chromosome t(2;8) juxtaposition; i.e., a locus derived from chromosome region 2q34 is attached to the 3' end of one MYC allele. The t(2;8) rearrangement in the HUT 78 cell line results in expression of a fused transcript encompassing the MYC gene and a locus designated TCL4 (T-cell leukemia/lymphoma 4), which normally resides on chromosome 2. The steady-state level of MYC-TCL4 fusion transcripts in HUT 78 cells is significantly higher than the MYC RNA level found in several other B- and T-cell lines. The production of fused MYC-TCL4 transcripts in a leukemic cell line raises the possibility that other B- and T-cell leukemias may express MYC fusion transcripts as an integral step in their pathogenesis.

AB - Primary cultures and established cell lines derived from human T-cell leukemias were analyzed for genomic rearrangements in the region 3' of the MYC locus. A T-cell leukemia line, HUT 78, whose 3' MYC region is rearranged, carries a chromosome t(2;8) juxtaposition; i.e., a locus derived from chromosome region 2q34 is attached to the 3' end of one MYC allele. The t(2;8) rearrangement in the HUT 78 cell line results in expression of a fused transcript encompassing the MYC gene and a locus designated TCL4 (T-cell leukemia/lymphoma 4), which normally resides on chromosome 2. The steady-state level of MYC-TCL4 fusion transcripts in HUT 78 cells is significantly higher than the MYC RNA level found in several other B- and T-cell lines. The production of fused MYC-TCL4 transcripts in a leukemic cell line raises the possibility that other B- and T-cell leukemias may express MYC fusion transcripts as an integral step in their pathogenesis.

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