Chromosomal assignment of a gene encoding a new collagen type (COL15A1) to 9q21 → q22

Kay Huebner, Linda A. Cannizzaro, Ethylin Wang Jabs, Sirpa Kiyirikko, Holly Manzone, Taina Pihlajaniemi, Jeanne C. Myers

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The collagens constitute a large family of extracellular matrix components primarily responsible for maintaining the structure and biological integrity of connective tissue. These proteins exhibit considerable diversity in size, sequence, tissue distribution, and molecular composition. Fourteen types of homo- and/or heterotrimeric molecules, thus far reported, are encoded by a minimum of 27 genes. Nineteen of these genes, including several that are closely linked, have been assigned to 10 separate autosomes, and one collagen gene has been mapped to the X chromosome. We have isolated a 2.1-kb human cDNA clone coding for a collagen molecule different in sequence and structure from types I-XIV collagens. This polypeptide has been designated the α1 chain of type XV collagen. To determine the location of the corresponding gene, the cDNA clone was hybridized to rodent-human hybrid DNAs and to human metaphase chromosomes. The results obtained using the hybrid cell lines showed that this newly identified collagen gene, COL15A1, is present in the pter → q34 region of chromosome 9. In situ hybridization allowed sublocalization to 9q21 → q22, a region to which no other collagen genes had previously been assigned. Our data further demonstrate the complex arrangement of the many collagen genes in the human genome.

Original languageEnglish (US)
Pages (from-to)220-224
Number of pages5
JournalGenomics
Volume14
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Collagen
Genes
Complementary DNA
Clone Cells
Chromosomes, Human, Pair 9
Hybrid Cells
Human Chromosomes
X Chromosome
Human Genome
Tissue Distribution
Metaphase
Collagen Type I
Connective Tissue
In Situ Hybridization
Extracellular Matrix
Rodentia
Cell Line
Peptides
DNA
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Huebner, K., Cannizzaro, L. A., Jabs, E. W., Kiyirikko, S., Manzone, H., Pihlajaniemi, T., & Myers, J. C. (1992). Chromosomal assignment of a gene encoding a new collagen type (COL15A1) to 9q21 → q22. Genomics, 14(2), 220-224. https://doi.org/10.1016/S0888-7543(05)80209-5

Chromosomal assignment of a gene encoding a new collagen type (COL15A1) to 9q21 → q22. / Huebner, Kay; Cannizzaro, Linda A.; Jabs, Ethylin Wang; Kiyirikko, Sirpa; Manzone, Holly; Pihlajaniemi, Taina; Myers, Jeanne C.

In: Genomics, Vol. 14, No. 2, 1992, p. 220-224.

Research output: Contribution to journalArticle

Huebner, K, Cannizzaro, LA, Jabs, EW, Kiyirikko, S, Manzone, H, Pihlajaniemi, T & Myers, JC 1992, 'Chromosomal assignment of a gene encoding a new collagen type (COL15A1) to 9q21 → q22', Genomics, vol. 14, no. 2, pp. 220-224. https://doi.org/10.1016/S0888-7543(05)80209-5
Huebner K, Cannizzaro LA, Jabs EW, Kiyirikko S, Manzone H, Pihlajaniemi T et al. Chromosomal assignment of a gene encoding a new collagen type (COL15A1) to 9q21 → q22. Genomics. 1992;14(2):220-224. https://doi.org/10.1016/S0888-7543(05)80209-5
Huebner, Kay ; Cannizzaro, Linda A. ; Jabs, Ethylin Wang ; Kiyirikko, Sirpa ; Manzone, Holly ; Pihlajaniemi, Taina ; Myers, Jeanne C. / Chromosomal assignment of a gene encoding a new collagen type (COL15A1) to 9q21 → q22. In: Genomics. 1992 ; Vol. 14, No. 2. pp. 220-224.
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