Chromogranin, an integral membrane protein

J. Settleman, J. Nolan, R. H. Angeletti

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Chromogranin is the major soluble protein of the adrenal medulla chromaffin granule and is secreted upon nervous stimulation. Using antisera to pure chromogranin in immunoblotting procedures, we show that chromogranin is the major integral membrane protein as well. Extraction of chromaffin granule membranes with low salt, high salt, chelating agents, or calcium-containing solutions does not remove the chromogranin from the membranes. The membrane form of chromogranin can be purified on a C-18 semipreparative column using high pressure liquid chromatography. Amino-terminal sequence data indicate that the membrane and soluble forms of chromogranin are identical or very similar.

Original languageEnglish (US)
Pages (from-to)1641-1644
Number of pages4
JournalJournal of Biological Chemistry
Volume260
Issue number3
StatePublished - 1985
Externally publishedYes

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Chromogranins
Membrane Proteins
Chromaffin Granules
Membranes
Salts
High pressure liquid chromatography
Adrenal Medulla
Chelating Agents
Immunoblotting
Immune Sera
High Pressure Liquid Chromatography
Calcium

ASJC Scopus subject areas

  • Biochemistry

Cite this

Settleman, J., Nolan, J., & Angeletti, R. H. (1985). Chromogranin, an integral membrane protein. Journal of Biological Chemistry, 260(3), 1641-1644.

Chromogranin, an integral membrane protein. / Settleman, J.; Nolan, J.; Angeletti, R. H.

In: Journal of Biological Chemistry, Vol. 260, No. 3, 1985, p. 1641-1644.

Research output: Contribution to journalArticle

Settleman, J, Nolan, J & Angeletti, RH 1985, 'Chromogranin, an integral membrane protein', Journal of Biological Chemistry, vol. 260, no. 3, pp. 1641-1644.
Settleman J, Nolan J, Angeletti RH. Chromogranin, an integral membrane protein. Journal of Biological Chemistry. 1985;260(3):1641-1644.
Settleman, J. ; Nolan, J. ; Angeletti, R. H. / Chromogranin, an integral membrane protein. In: Journal of Biological Chemistry. 1985 ; Vol. 260, No. 3. pp. 1641-1644.
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