Chromogranin A and B in adenomas of the pituitary: An immunohistochemical study of 42 cases

K. W. Schmid, M. Kroll, A. Hittmair, H. Maier, M. Totsch, R. Gasser, G. Finkenstett, R. Hogue-Angeletti, R. Fischer-Colbrie

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Abstract

Forty-two pituitary adenomas (10 prolactinomas; three ACTH-, nine GH-, two FSH- and two TSH-secreting adenomas; and 16 clinically nonfunctioning null cell adenomas) were investigated immunohistochemically with antibodies against chromogranin A and B as well as ACTH, GH, prolactin (PRL), FSH, LH, TSH, and α-HCG antibodies. For the demonstration of chromogranin B, two different antibodies were used-e.g., a polyclonal antihuman antibody and an antiserum against a synthetic peptide (DK-21, chromogranin B 306-326) present in the chromogranin B amino acid sequence. All tumors were positive for both chromogranin B antibodies. Chromogranin A was found in FSH- (two of two) and TSH- (two of two) secreting adenomas; it was also found in a focal distribution in ACTH- (one of three) and GH- (four of nine) secreting adenomas. Thirteen of 16 null cell adenomas contained chromogranin A, whereas no chromogranin A was found in prolactinomas. We conclude that null cell adenomas may arise either from FSH/LH or TSH cells (null cell adenomas with both chromogranin A and B positivity) or from ACTH, GH, or PRL cells (the respective tumors are only positive for chromogranin B). Chromogranin B may be used as a universal marker for pituitary adenomas.

Original languageEnglish (US)
Pages (from-to)1072-1077
Number of pages6
JournalAmerican Journal of Surgical Pathology
Volume15
Issue number11
StatePublished - 1991
Externally publishedYes

Fingerprint

Chromogranin B
Chromogranin A
Pituitary Neoplasms
Adenoma
Null Lymphocytes
Adrenocorticotropic Hormone
Antibodies
Gonadotrophs
Prolactinoma
Prolactin
Thyrotrophs
Immune Sera
Amino Acid Sequence
Neoplasms

Keywords

  • Chromogranin A and B
  • Pituitary
  • Pituitary adenomas

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Schmid, K. W., Kroll, M., Hittmair, A., Maier, H., Totsch, M., Gasser, R., ... Fischer-Colbrie, R. (1991). Chromogranin A and B in adenomas of the pituitary: An immunohistochemical study of 42 cases. American Journal of Surgical Pathology, 15(11), 1072-1077.

Chromogranin A and B in adenomas of the pituitary : An immunohistochemical study of 42 cases. / Schmid, K. W.; Kroll, M.; Hittmair, A.; Maier, H.; Totsch, M.; Gasser, R.; Finkenstett, G.; Hogue-Angeletti, R.; Fischer-Colbrie, R.

In: American Journal of Surgical Pathology, Vol. 15, No. 11, 1991, p. 1072-1077.

Research output: Contribution to journalArticle

Schmid, KW, Kroll, M, Hittmair, A, Maier, H, Totsch, M, Gasser, R, Finkenstett, G, Hogue-Angeletti, R & Fischer-Colbrie, R 1991, 'Chromogranin A and B in adenomas of the pituitary: An immunohistochemical study of 42 cases', American Journal of Surgical Pathology, vol. 15, no. 11, pp. 1072-1077.
Schmid KW, Kroll M, Hittmair A, Maier H, Totsch M, Gasser R et al. Chromogranin A and B in adenomas of the pituitary: An immunohistochemical study of 42 cases. American Journal of Surgical Pathology. 1991;15(11):1072-1077.
Schmid, K. W. ; Kroll, M. ; Hittmair, A. ; Maier, H. ; Totsch, M. ; Gasser, R. ; Finkenstett, G. ; Hogue-Angeletti, R. ; Fischer-Colbrie, R. / Chromogranin A and B in adenomas of the pituitary : An immunohistochemical study of 42 cases. In: American Journal of Surgical Pathology. 1991 ; Vol. 15, No. 11. pp. 1072-1077.
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AU - Maier, H.

AU - Totsch, M.

AU - Gasser, R.

AU - Finkenstett, G.

AU - Hogue-Angeletti, R.

AU - Fischer-Colbrie, R.

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