Chromatin signature

Brandon J. Thomas, Eric D. Rubio, Niklas Krumm, Pilib Broin, Karol Bomsztyk, Piri Welcsh, John M. Greally, Aaron A.J. Golden, Anton Krumm

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Allele-specific gene expression is an integral component of cellular programming and development and contributes to the diversity of cellular phenotypes [1, 2]. Allelic differences in gene expression are mediated by either parent-of-origin-specific selection (imprinting) or stochastic selection of alleles for activation and/or silencing. The importance of genomic imprinting has recently been highlighted by RNA sequencing studies that demonstrated widespread allelic differences in gene expression in mouse brain affecting more than 1,300 genes [3]. The extent of sex-and stagespecific expression of individual alleles emphasizes the essential role of allelic transcriptional regulation in development. In addition to the extensive occurrence of imprinted parent-of-origin-specific expression, gene expression patterns of clonal cell populations are also modified by random or stochastic silencing of either the maternal or paternal allele. Wellknown loci displaying allele-specific expression include odorant receptor genes, immunoglobulins and various receptor proteins [4-6]. Additionally, previous large-scale studies have provided new data demonstrating that parent-of-origin-specific expression is employed much more frequently than previously thought [7]. These new findings illustrate the scale and complexity of genomic allele-specific expression. However, the precise molecular mechanism underlying the allelic bias in gene expression is not very well understood.

Original languageEnglish (US)
Title of host publicationEpigenetics, Environment, and Genes
PublisherApple Academic Press
Pages207-239
Number of pages33
ISBN (Electronic)9781482216660
ISBN (Print)9781926895253
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Keywords

  • Alleles
  • Homozygous
  • Immunoprecipitation
  • Lysine 9
  • Oligonucleotide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Thomas, B. J., Rubio, E. D., Krumm, N., Broin, P., Bomsztyk, K., Welcsh, P., Greally, J. M., Golden, A. A. J., & Krumm, A. (2013). Chromatin signature. In Epigenetics, Environment, and Genes (pp. 207-239). Apple Academic Press. https://doi.org/10.1201/b14910