Chromatin organization at the nuclear pore favours HIV replication

Mickaël Lelek, Nicoletta Casartelli, Danilo Pellin, Ermanno Rizzi, Philippe Souque, Marco Severgnini, Clelia Di Serio, Thomas Fricke, Felipe Diaz-Griffero, Christophe Zimmer, Pierre Charneau, Francesca Di Nunzio

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The molecular mechanisms that allow HIV to integrate into particular sites of the host genome are poorly understood. Here we tested if the nuclear pore complex (NPC) facilitates the targeting of HIV integration by acting on chromatin topology. We show that the integrity of the nuclear side of the NPC, which is mainly composed of Tpr, is not required for HIV nuclear import, but that Nup153 is essential. Depletion of Tpr markedly reduces HIV infectivity, but not the level of integration. HIV integration sites in Tpr-depleted cells are less associated with marks of active genes, consistent with the state of chromatin proximal to the NPC, as analysed by super-resolution microscopy. LEDGF/p75, which promotes viral integration into active genes, stabilizes Tpr at the nuclear periphery and vice versa. Our data support a model in which HIV nuclear import and integration are concerted steps, and where Tpr maintains a chromatin environment favourable for HIV replication.

Original languageEnglish (US)
Article number7483
JournalNature Communications
Volume6
DOIs
StatePublished - 2015

Fingerprint

Nuclear Pore
chromatin
human immunodeficiency virus
Chromatin
HIV
porosity
Genes
Cell Nucleus Active Transport
genes
Microscopic examination
Virus Integration
Topology
genome
integrity
Microscopy
depletion
topology
Genome
microscopy
cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Lelek, M., Casartelli, N., Pellin, D., Rizzi, E., Souque, P., Severgnini, M., ... Di Nunzio, F. (2015). Chromatin organization at the nuclear pore favours HIV replication. Nature Communications, 6, [7483]. https://doi.org/10.1038/ncomms7483

Chromatin organization at the nuclear pore favours HIV replication. / Lelek, Mickaël; Casartelli, Nicoletta; Pellin, Danilo; Rizzi, Ermanno; Souque, Philippe; Severgnini, Marco; Di Serio, Clelia; Fricke, Thomas; Diaz-Griffero, Felipe; Zimmer, Christophe; Charneau, Pierre; Di Nunzio, Francesca.

In: Nature Communications, Vol. 6, 7483, 2015.

Research output: Contribution to journalArticle

Lelek, M, Casartelli, N, Pellin, D, Rizzi, E, Souque, P, Severgnini, M, Di Serio, C, Fricke, T, Diaz-Griffero, F, Zimmer, C, Charneau, P & Di Nunzio, F 2015, 'Chromatin organization at the nuclear pore favours HIV replication', Nature Communications, vol. 6, 7483. https://doi.org/10.1038/ncomms7483
Lelek M, Casartelli N, Pellin D, Rizzi E, Souque P, Severgnini M et al. Chromatin organization at the nuclear pore favours HIV replication. Nature Communications. 2015;6. 7483. https://doi.org/10.1038/ncomms7483
Lelek, Mickaël ; Casartelli, Nicoletta ; Pellin, Danilo ; Rizzi, Ermanno ; Souque, Philippe ; Severgnini, Marco ; Di Serio, Clelia ; Fricke, Thomas ; Diaz-Griffero, Felipe ; Zimmer, Christophe ; Charneau, Pierre ; Di Nunzio, Francesca. / Chromatin organization at the nuclear pore favours HIV replication. In: Nature Communications. 2015 ; Vol. 6.
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