Chromatin immunoprecipitation in adult zebrafish red cells

Eirini Trompouki, Teresa V. Bowman, Anthony DiBiase, Yi Zhou, Leonard I. Zon

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Zebrafish has been used for many years as a model to study development and disease. The ability of zebrafish to produce thousand of embryos in a synchronous manner has made zebrafish an invaluable tool for genetic and chemical screens. Since its emergence as an important model organism the molecular tools for studying zebrafish have been limited. In this chapter, we describe a simple method to identify DNA binding sites and chromatin architecture in erythrocytes from adult zebrafish using chromatin immunoprecipitation coupled with next generation sequencing. This technique has been used extensively and successfully in other systems and it will be a useful tool for studying epigenetics in zebrafish.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages341-352
Number of pages12
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Cell Biology
Volume104
ISSN (Print)0091-679X

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Keywords

  • Adult erythrocytes
  • Chromatin immunoprecipitation
  • High throughput sequencing
  • Transcription factor
  • Zebrafish

ASJC Scopus subject areas

  • Cell Biology

Cite this

Trompouki, E., Bowman, T. V., DiBiase, A., Zhou, Y., & Zon, L. I. (2011). Chromatin immunoprecipitation in adult zebrafish red cells. In Methods in Cell Biology (pp. 341-352). (Methods in Cell Biology; Vol. 104). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-374814-0.00019-7