Chromatin assembly using Drosophila systems.

Dmitry Fyodorov, Mark E. Levenstein

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To successfully study chromatin structure and activity in vitro, it is essential to have a chromatin assembly system that will prepare extended nucleosome arrays with highly defined protein content that resemble bulk chromatin isolated from living cell nuclei in terms of periodicity and nucleosome positioning. The Drosophila ATP-dependent chromatin assembly system described in this unit meets these requirements. The end product of the reaction described here has highly periodic extended arrays with physiologic spacing and positioning of the nucleosomes.

Original languageEnglish (US)
JournalCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
VolumeChapter 21
StatePublished - May 2002
Externally publishedYes

Fingerprint

Chromatin Assembly and Disassembly
Nucleosomes
Drosophila
Chromatin
Periodicity
Cell Nucleus
Adenosine Triphosphate
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chromatin assembly using Drosophila systems. / Fyodorov, Dmitry; Levenstein, Mark E.

In: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Vol. Chapter 21, 05.2002.

Research output: Contribution to journalArticle

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