Chromaffin granule and PC12 cell chondroitin sulfate proteoglycans and their relation to chromogranin A

D. C. Gowda, R. Hogue-Angeletti, R. K. Margolis, R. U. Margolis

Research output: Contribution to journalArticle

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Abstract

Two major proteoglycans, which appear to be structurally closely related, were isolated from bovine chromaffin granule matrix proteins by ion-exchange chromatography. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis they have apparent average molecular sizes of 35-40 kDa (range of 23-75 kDa) and generate a 14-kDa core glycoprotein after chondroitinase treatment. Previous studies demonstrated that these two major chromaffin granule proteoglycans are very similar in terms of their peptide mapping patterns and carbohydrate composition (having a high proportion of tri- and tetraantennary N-glycosidic oligosaccharides, and O-glycosidic oligosaccharides consisting predominantly of disialyl derivatives of galactosyl(β1-3)N-acetylgalactosamine), and that they differed in these respects from the chromogranins. By using antisera to five synthetic peptide fragments of chromogranin A to stain immunoblots of purified chromaffin granule proteoglycans before and after chondroitinase treatment, we have now shown that these major proteoglycans are not immunochemically related to chromogranin A. However, it has recently been reported that some chromogranin A-immunoreactive material disappears after chondroitinase treatment, and our studies demonstrate that approximately 1-2% of the chromogranin A occurs in the form of a 110-kDa proteoglycan, which is converted to a 95-kDa core glycoprotein after chondroitinase treatment. Similar chromogranin A proteoglycans could be detected in rat PC12 pheochromocytoma cells, where they have a molecular size of 115-145 kDa and yield a 105-kDa core protein after chondroitinase treatment. Studies using antibodies to synthetic peptide fragments of chromogranin B (secretogranin I) did not provide any evidence that this related protein occurs in a proteoglycan form.

Original languageEnglish (US)
Pages (from-to)219-224
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume281
Issue number2
DOIs
StatePublished - 1990

Fingerprint

Chromaffin Granules
Chondroitin Sulfate Proteoglycans
Chromogranin A
Chromaffin Cells
PC12 Cells
Chondroitinases and Chondroitin Lyases
Proteoglycans
Chromogranin B
Peptide Fragments
Oligosaccharides
Glycoproteins
Chromogranins
Acetylgalactosamine
Proteins
Peptide Mapping
Ion Exchange Chromatography
Pheochromocytoma
Chromatography
Electrophoresis
Sodium Dodecyl Sulfate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chromaffin granule and PC12 cell chondroitin sulfate proteoglycans and their relation to chromogranin A. / Gowda, D. C.; Hogue-Angeletti, R.; Margolis, R. K.; Margolis, R. U.

In: Archives of Biochemistry and Biophysics, Vol. 281, No. 2, 1990, p. 219-224.

Research output: Contribution to journalArticle

Gowda, D. C. ; Hogue-Angeletti, R. ; Margolis, R. K. ; Margolis, R. U. / Chromaffin granule and PC12 cell chondroitin sulfate proteoglycans and their relation to chromogranin A. In: Archives of Biochemistry and Biophysics. 1990 ; Vol. 281, No. 2. pp. 219-224.
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