CHoMP: A chemoenzymatic histology method using clickable probes

Sara H. Rouhanifard, Aimé López-Aguilar, Peng Wu

Research output: Contribution to journalReview article

10 Scopus citations

Abstract

The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for detection. Here, we report the development of a histological method, dubbed CHoMP-chemoenzymatic histology of membrane polysaccharides-for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analogue equipped with a chemical handle to a specific cell-surface glycan target, which can then be derivatized with imaging probes by using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N-acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, thus suggesting a potential application of this technique in early cancer diagnosis.

Original languageEnglish (US)
Pages (from-to)2667-2673
Number of pages7
JournalChemBioChem
Volume15
Issue number18
DOIs
Publication statusPublished - Nov 17 2014

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Cite this

Rouhanifard, S. H., López-Aguilar, A., & Wu, P. (2014). CHoMP: A chemoenzymatic histology method using clickable probes. ChemBioChem, 15(18), 2667-2673. https://doi.org/10.1002/cbic.201402433