CHoMP: A chemoenzymatic histology method using clickable probes

Sara H. Rouhanifard, Aimé López-Aguilar, Peng Wu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for detection. Here, we report the development of a histological method, dubbed CHoMP-chemoenzymatic histology of membrane polysaccharides-for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analogue equipped with a chemical handle to a specific cell-surface glycan target, which can then be derivatized with imaging probes by using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N-acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, thus suggesting a potential application of this technique in early cancer diagnosis.

Original languageEnglish (US)
Pages (from-to)2667-2673
Number of pages7
JournalChemBioChem
Volume15
Issue number18
DOIs
StatePublished - Nov 17 2014

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Glycosylation
Histology
Polysaccharides
Glycosyltransferases
Monosaccharides
Click Chemistry
Tumors
Visualization
Tissue
Membranes
Early Detection of Cancer
Imaging techniques
Neoplasms
N-acetyllactosamine

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology

Cite this

Rouhanifard, S. H., López-Aguilar, A., & Wu, P. (2014). CHoMP: A chemoenzymatic histology method using clickable probes. ChemBioChem, 15(18), 2667-2673. https://doi.org/10.1002/cbic.201402433

CHoMP : A chemoenzymatic histology method using clickable probes. / Rouhanifard, Sara H.; López-Aguilar, Aimé; Wu, Peng.

In: ChemBioChem, Vol. 15, No. 18, 17.11.2014, p. 2667-2673.

Research output: Contribution to journalArticle

Rouhanifard, SH, López-Aguilar, A & Wu, P 2014, 'CHoMP: A chemoenzymatic histology method using clickable probes', ChemBioChem, vol. 15, no. 18, pp. 2667-2673. https://doi.org/10.1002/cbic.201402433
Rouhanifard, Sara H. ; López-Aguilar, Aimé ; Wu, Peng. / CHoMP : A chemoenzymatic histology method using clickable probes. In: ChemBioChem. 2014 ; Vol. 15, No. 18. pp. 2667-2673.
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