Cholesterol Esterification by Host and Parasite Is Essential for Optimal Proliferation of Toxoplasma gondii

Sabrina Sonda, Li Min Ting, Sabine Novak, Kami Kim, Jacquelyn J. Maher, Robert V. Farese, Joel D. Ernst

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Upon host cell invasion the apicomplexan parasite Toxoplasma gondii resides in a specialized compartment termed the parasitophorous vacuole that is derived from the host cell membrane but modified by the parasite. Despite the segregation of the parasitophorous vacuole from the host endocytic network, the intravacuolar parasite has been shown to acquire cholesterol from the host cell. In order to characterize further the role of sterol metabolism in T. gondii biology, we focused our studies on the activity of acyl-CoA:cholesterol acyltransferase (ACAT), a key enzyme for maintaining the intracellular homeostasis of cholesterol through the formation of cholesterol esters. In this study, we demonstrate that ACAT and cholesterol esters play a crucial role in the optimal replication of T. gondii. Moreover, we identified ACAT activity in T. gondii that can be modulated by pharmacological ACAT inhibitors with a consequent detrimental effect on parasite replication.

Original languageEnglish (US)
Pages (from-to)34434-34440
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number37
DOIs
StatePublished - Sep 14 2001
Externally publishedYes

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Sterol O-Acyltransferase
Esterification
Toxoplasma
Parasites
Cholesterol
Cholesterol Esters
Vacuoles
Anticholesteremic Agents
Sterols
Cell membranes
Metabolism
Homeostasis
Cell Membrane
Pharmacology
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sonda, S., Ting, L. M., Novak, S., Kim, K., Maher, J. J., Farese, R. V., & Ernst, J. D. (2001). Cholesterol Esterification by Host and Parasite Is Essential for Optimal Proliferation of Toxoplasma gondii. Journal of Biological Chemistry, 276(37), 34434-34440. https://doi.org/10.1074/jbc.M105025200

Cholesterol Esterification by Host and Parasite Is Essential for Optimal Proliferation of Toxoplasma gondii. / Sonda, Sabrina; Ting, Li Min; Novak, Sabine; Kim, Kami; Maher, Jacquelyn J.; Farese, Robert V.; Ernst, Joel D.

In: Journal of Biological Chemistry, Vol. 276, No. 37, 14.09.2001, p. 34434-34440.

Research output: Contribution to journalArticle

Sonda, S, Ting, LM, Novak, S, Kim, K, Maher, JJ, Farese, RV & Ernst, JD 2001, 'Cholesterol Esterification by Host and Parasite Is Essential for Optimal Proliferation of Toxoplasma gondii', Journal of Biological Chemistry, vol. 276, no. 37, pp. 34434-34440. https://doi.org/10.1074/jbc.M105025200
Sonda, Sabrina ; Ting, Li Min ; Novak, Sabine ; Kim, Kami ; Maher, Jacquelyn J. ; Farese, Robert V. ; Ernst, Joel D. / Cholesterol Esterification by Host and Parasite Is Essential for Optimal Proliferation of Toxoplasma gondii. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 37. pp. 34434-34440.
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