Cholecystokinin inhibits gastric emptying and contracts the pyloric sphincter in rats by interacting with low affinity CCK receptor sites

Timothy H. Moran, Rebecca Kornbluh, Kimberly Moore, Gary J. Schwartz

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The aim of these experiments was to characterize the receptor affinity state through which CCK produces pyloric contraction and inhibits gastric emptying in the rat using the novel CCK heptapeptide analog CCK-JMV-180. CCK-JMV-180 has been demonstrated to act as a functional agonist at high affinity pancreatic CCKA receptors but as a functional antagonist at CCKA low affinity receptors. CCK-8 (1, 3.2 and 10 nM) induced dose dependent tension increases in isolated pyloric segments. CCK-JMV-180 (3.2 μM) or vehicle failed to mimic this action when administered alone but blocked the ability of CCK-8 (3.2 nM) to induce tension increases. CCK-8 (2 μg/kg) also inhibited the gastric emptying of physiological saline. CCK-JMV-180 (320 and 1000 μg/kg) failed to inhibit emptying when administered alone but dose dependently antagonized CCK induced inhibition of gastric emptying. Thus, in both preparations CCK-JMV-180 acted as a functional CCK antagonist. This profile is consistent with the interpretation that the actions of CCK in pyloric contraction and the inhibition of gastric emptying are mediated through CCK's interactions with receptors functionally similar to pancreatic low affinity sites.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalRegulatory Peptides
Volume52
Issue number3
DOIs
StatePublished - Aug 4 1994
Externally publishedYes

Keywords

  • CCK-JMV-180
  • Neuropeptide

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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