TY - JOUR
T1 - Chitosan-based thermosensitive composite hydrogel enhances the therapeutic efficacy of human umbilical cord MSC in TBI rat model
AU - Yao, M.
AU - Chen, Y.
AU - Zhang, J.
AU - Gao, F.
AU - Ma, S.
AU - Guan, F.
N1 - Funding Information:
The project was supported by the National Natural Science Foundation of China ( NSFC 31700820 ), Joint Fund of the National Natural Science Foundation of China and Henan province ( U1804198 ), China Postdoctoral Science Foundation ( 2017M612420 ), and Key Scientific Research Projects of higher education institutions in Henan province ( 18A180003 ).
PY - 2019/12
Y1 - 2019/12
N2 - Recently, stem cell–based therapy shows great promise in treating traumatic brain injury. However, the low rate of cell engraftment and survival are two major barriers for efficacy. To improve the therapeutic effect, a new thermosensitive hydrogel based on chitosan, hydroxyethyl cellulose, hyaluronic acid, and β-glycerophosphate (CS-HEC-HA/GP) was developed in this study. This CS-HEC-HA/GP hydrogel exhibits a faster gelation process and better biocompatibility to human umbilical cord mesenchymal stem cells (hUC-MSC) versus CS/GP or CS-HEC/GP hydrogels. The suitable rheological behavior similar to brain tissue supports that the CS-HEC-HA/GP hydrogel might be a preferable neural scaffold. In addition, CS-HEC-HA/GP hydrogel loaded with hUC-MSC could enhance the retention, survival, and migration of encapsulated hUC-MSC, improve survival and proliferation of endogenous neural cells probably by secreting neurotrophic factors and inhibiting apoptosis, and thereby accelerate remodeling of brain structure and neurological function recovery in TBI rats. Thus, this hydrogel shows enormous potentials in stem cell–based neural tissue repair and regeneration.
AB - Recently, stem cell–based therapy shows great promise in treating traumatic brain injury. However, the low rate of cell engraftment and survival are two major barriers for efficacy. To improve the therapeutic effect, a new thermosensitive hydrogel based on chitosan, hydroxyethyl cellulose, hyaluronic acid, and β-glycerophosphate (CS-HEC-HA/GP) was developed in this study. This CS-HEC-HA/GP hydrogel exhibits a faster gelation process and better biocompatibility to human umbilical cord mesenchymal stem cells (hUC-MSC) versus CS/GP or CS-HEC/GP hydrogels. The suitable rheological behavior similar to brain tissue supports that the CS-HEC-HA/GP hydrogel might be a preferable neural scaffold. In addition, CS-HEC-HA/GP hydrogel loaded with hUC-MSC could enhance the retention, survival, and migration of encapsulated hUC-MSC, improve survival and proliferation of endogenous neural cells probably by secreting neurotrophic factors and inhibiting apoptosis, and thereby accelerate remodeling of brain structure and neurological function recovery in TBI rats. Thus, this hydrogel shows enormous potentials in stem cell–based neural tissue repair and regeneration.
KW - Human umbilical cord mesenchymal stem cells
KW - Neurological function recovery
KW - Thermosensitive hydrogel
KW - Traumatic brain injury
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U2 - 10.1016/j.mtchem.2019.08.011
DO - 10.1016/j.mtchem.2019.08.011
M3 - Article
AN - SCOPUS:85072269141
VL - 14
JO - Materials Today Chemistry
JF - Materials Today Chemistry
SN - 2468-5194
M1 - 100192
ER -