Chemotactic activity of the peroxidized retinal membrane lipids in experimental autoimmune uveitis

Hiroshi Goto; Guey Shuang Wu; David C. Gritz; Lily R. Atalla; Narsing A. Rao

doi:10.3109/02713689109020339

Chemotactic activity of the peroxidized retinal membrane lipids in experimental autoimmune uveitis

Hiroshi Goto, Guey Shuang Wu, David C. Gritz, Lily R. Atalla, Narsing A. Rao

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We investigated the mechanism for amplification of intraocular inflammation in rats with experimental autoimmune uveitis by examining the chemotaxis potentials of peroxidized lipids extracted from the retinas. Utilizing thin layer chromatography, we found that the peroxidized products isolated from the inflamed retinas were fatty acid hydroperoxides that corresponded to the autooxidized products from commercial methyl docosahexaenoate, with Rf values ranging from 0.30 to 0.37. These were not demonstrated in similar preparations from normal retinas or in unoxidized docosahexaenoate. Boyden chamber assay revealed that the hydroperoxides isolated from inflamed eyes and the products of oxidized methyl docosahexaenoate possessed significantly higher chemotactic activity than did the retinal lipids isolated from normal eyes (P < 0.01). These findings may help to explain the mechanism of inflammatory amplification induced by peroxidized retinal lipids that is seen in this animal model of uveitis.

Original language	English (US)
Pages (from-to)	1009-1014
Number of pages	6
Journal	Current Eye Research
Volume	10
Issue number	11
DOIs	https://doi.org/10.3109/02713689109020339
State	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "Chemotactic activity of the peroxidized retinal membrane lipids in experimental autoimmune uveitis",

abstract = "We investigated the mechanism for amplification of intraocular inflammation in rats with experimental autoimmune uveitis by examining the chemotaxis potentials of peroxidized lipids extracted from the retinas. Utilizing thin layer chromatography, we found that the peroxidized products isolated from the inflamed retinas were fatty acid hydroperoxides that corresponded to the autooxidized products from commercial methyl docosahexaenoate, with Rf values ranging from 0.30 to 0.37. These were not demonstrated in similar preparations from normal retinas or in unoxidized docosahexaenoate. Boyden chamber assay revealed that the hydroperoxides isolated from inflamed eyes and the products of oxidized methyl docosahexaenoate possessed significantly higher chemotactic activity than did the retinal lipids isolated from normal eyes (P < 0.01). These findings may help to explain the mechanism of inflammatory amplification induced by peroxidized retinal lipids that is seen in this animal model of uveitis.",

author = "Hiroshi Goto and Wu, {Guey Shuang} and Gritz, {David C.} and Atalla, {Lily R.} and Rao, {Narsing A.}",

note = "Funding Information: ACKNOWLEDGMENTS Supported in part by grant EY 05662 from the National Institutes of Health and by an unrestricted grant from Research to Prevent Blindness, Inc., New York, NY. Ann Dawson, medical editor, reviewed this manuscript.",

year = "1991",

doi = "10.3109/02713689109020339",

language = "English (US)",

volume = "10",

pages = "1009--1014",

journal = "Current Eye Research",

issn = "0271-3683",

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T1 - Chemotactic activity of the peroxidized retinal membrane lipids in experimental autoimmune uveitis

AU - Goto, Hiroshi

AU - Wu, Guey Shuang

AU - Gritz, David C.

AU - Atalla, Lily R.

AU - Rao, Narsing A.

N1 - Funding Information: ACKNOWLEDGMENTS Supported in part by grant EY 05662 from the National Institutes of Health and by an unrestricted grant from Research to Prevent Blindness, Inc., New York, NY. Ann Dawson, medical editor, reviewed this manuscript.

PY - 1991

Y1 - 1991

N2 - We investigated the mechanism for amplification of intraocular inflammation in rats with experimental autoimmune uveitis by examining the chemotaxis potentials of peroxidized lipids extracted from the retinas. Utilizing thin layer chromatography, we found that the peroxidized products isolated from the inflamed retinas were fatty acid hydroperoxides that corresponded to the autooxidized products from commercial methyl docosahexaenoate, with Rf values ranging from 0.30 to 0.37. These were not demonstrated in similar preparations from normal retinas or in unoxidized docosahexaenoate. Boyden chamber assay revealed that the hydroperoxides isolated from inflamed eyes and the products of oxidized methyl docosahexaenoate possessed significantly higher chemotactic activity than did the retinal lipids isolated from normal eyes (P < 0.01). These findings may help to explain the mechanism of inflammatory amplification induced by peroxidized retinal lipids that is seen in this animal model of uveitis.

AB - We investigated the mechanism for amplification of intraocular inflammation in rats with experimental autoimmune uveitis by examining the chemotaxis potentials of peroxidized lipids extracted from the retinas. Utilizing thin layer chromatography, we found that the peroxidized products isolated from the inflamed retinas were fatty acid hydroperoxides that corresponded to the autooxidized products from commercial methyl docosahexaenoate, with Rf values ranging from 0.30 to 0.37. These were not demonstrated in similar preparations from normal retinas or in unoxidized docosahexaenoate. Boyden chamber assay revealed that the hydroperoxides isolated from inflamed eyes and the products of oxidized methyl docosahexaenoate possessed significantly higher chemotactic activity than did the retinal lipids isolated from normal eyes (P < 0.01). These findings may help to explain the mechanism of inflammatory amplification induced by peroxidized retinal lipids that is seen in this animal model of uveitis.

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Chemotactic activity of the peroxidized retinal membrane lipids in experimental autoimmune uveitis

Abstract

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