Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives

David Soriano del Amo, Wei Wang, Christen Besanceney, Tianqing Zheng, Yizheng He, Brian Gerwe, Ronald D. Seidel, Peng Wu

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A combination of recombinant FKP and α-(1→3)-fucosyltransferase allows the facile synthesis of the sialyl Lewis X tetrasaccharide glycan and its derivatives in excellent yield. In this system, the universal fucosyl donor, guanidine 5′-diphosphate-β-l-fucose (GDP-fucose), or its analogues can be generated in situ by cofactor recycling using pyruvate kinase.

Original languageEnglish (US)
Pages (from-to)1107-1113
Number of pages7
JournalCarbohydrate Research
Volume345
Issue number9
DOIs
StatePublished - Jun 16 2010

Keywords

  • Chemoenzymatic synthesis
  • Fucose
  • Sialic acid
  • Transferases

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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