Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives

David Soriano del Amo, Wei Wang, Christen Besanceney, Tianqing Zheng, Yizheng He, Brian Gerwe, Ronald D. Seidel, Peng Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A combination of recombinant FKP and α-(1→3)-fucosyltransferase allows the facile synthesis of the sialyl Lewis X tetrasaccharide glycan and its derivatives in excellent yield. In this system, the universal fucosyl donor, guanidine 5′-diphosphate-β-l-fucose (GDP-fucose), or its analogues can be generated in situ by cofactor recycling using pyruvate kinase.

Original languageEnglish (US)
Pages (from-to)1107-1113
Number of pages7
JournalCarbohydrate Research
Volume345
Issue number9
DOIs
StatePublished - Jun 16 2010

Fingerprint

Fucosyltransferases
Pyruvate Kinase
Fucose
Diphosphates
Guanidine
Recycling
Polysaccharides
Derivatives

Keywords

  • Chemoenzymatic synthesis
  • Fucose
  • Sialic acid
  • Transferases

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Organic Chemistry
  • Medicine(all)

Cite this

Soriano del Amo, D., Wang, W., Besanceney, C., Zheng, T., He, Y., Gerwe, B., ... Wu, P. (2010). Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives. Carbohydrate Research, 345(9), 1107-1113. https://doi.org/10.1016/j.carres.2010.03.032

Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives. / Soriano del Amo, David; Wang, Wei; Besanceney, Christen; Zheng, Tianqing; He, Yizheng; Gerwe, Brian; Seidel, Ronald D.; Wu, Peng.

In: Carbohydrate Research, Vol. 345, No. 9, 16.06.2010, p. 1107-1113.

Research output: Contribution to journalArticle

Soriano del Amo, D, Wang, W, Besanceney, C, Zheng, T, He, Y, Gerwe, B, Seidel, RD & Wu, P 2010, 'Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives', Carbohydrate Research, vol. 345, no. 9, pp. 1107-1113. https://doi.org/10.1016/j.carres.2010.03.032
Soriano del Amo D, Wang W, Besanceney C, Zheng T, He Y, Gerwe B et al. Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives. Carbohydrate Research. 2010 Jun 16;345(9):1107-1113. https://doi.org/10.1016/j.carres.2010.03.032
Soriano del Amo, David ; Wang, Wei ; Besanceney, Christen ; Zheng, Tianqing ; He, Yizheng ; Gerwe, Brian ; Seidel, Ronald D. ; Wu, Peng. / Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives. In: Carbohydrate Research. 2010 ; Vol. 345, No. 9. pp. 1107-1113.
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