Chemoenzymatic Synthesis of 36S Isotopologues of Methionine and S-Adenosyl-L-methionine

Myles B. Poulin, Quan Du, Vern L. Schramm

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Abstract

Substrates containing isotope labels at specific atoms are required for transition-state analysis based on the measurement of multiple kinetic isotope effects.36S-labeled L-methionine and S-adenosyl-L-methionine were synthesized from elemental sulfur using a chemoenzymatic approach with >98% 36S enrichment. This method provides access to previously inaccessible sulfur isotope-labeled substrates for sulfur kinetic isotope effect studies. (Chemical Equation Presented).

Original languageEnglish (US)
Pages (from-to)5344-5347
Number of pages4
JournalJournal of Organic Chemistry
Volume80
Issue number10
DOIs
Publication statusPublished - May 15 2015

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ASJC Scopus subject areas

  • Organic Chemistry

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