Chemoenzymatic Synthesis of <sup>36</sup>S Isotopologues of Methionine and S-Adenosyl-L-methionine

Myles B. Poulin, Quan Du, Vern L. Schramm

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Substrates containing isotope labels at specific atoms are required for transition-state analysis based on the measurement of multiple kinetic isotope effects.<sup>36</sup>S-labeled L-methionine and S-adenosyl-L-methionine were synthesized from elemental sulfur using a chemoenzymatic approach with >98% <sup>36</sup>S enrichment. This method provides access to previously inaccessible sulfur isotope-labeled substrates for sulfur kinetic isotope effect studies. (Chemical Equation Presented).

Original languageEnglish (US)
Pages (from-to)5344-5347
Number of pages4
JournalJournal of Organic Chemistry
Volume80
Issue number10
DOIs
StatePublished - May 15 2015

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Sulfur Isotopes
S-Adenosylmethionine
Isotopes
Kinetics
Substrates
Sulfur
Labels
Atoms

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Chemoenzymatic Synthesis of <sup>36</sup>S Isotopologues of Methionine and S-Adenosyl-L-methionine. / Poulin, Myles B.; Du, Quan; Schramm, Vern L.

In: Journal of Organic Chemistry, Vol. 80, No. 10, 15.05.2015, p. 5344-5347.

Research output: Contribution to journalArticle

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