Chemoattractant-induced lamellipod extension

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The mammary adenocarcinoma cell line MTLn3 is chemotactic towards epidermal growth factor (EGF), and this induced motility is thought to promote breast cancer invasion and metastasis. Stimulation of MTLn3 cells with EGF results in the extension of a flat, thin structure filled with filamentous actin and termed a lamellipod. Lamellipod extension is dependent on actin polymerization and is localized to the border of adherent cells. The structure of EGF-stimulated lamellipods in MTLn3 cells is well suited to analysis of chemoattractant-stimulated protrusion. Actin polymerization occurs within 200 nm of the extending edge of the lamellipod. Although extension of the lamellipod is not dependent upon interaction with the substratum, stabilization of the extended lamellipod is dependent on an adhesive substratum. Dorsal ruffling is suppressed during lamellipod extension. Tyrosine phosphorylation is reduced in preexisting focal contacts compared to new contacts induced by EGF stimulation. The coordination of turnover of focal contacts with lamellipod extension is proposed to result in polarized cell motility in response to gradients of chemoattractants.

Original languageEnglish (US)
Pages (from-to)433-443
Number of pages11
JournalMicroscopy Research and Technique
Volume43
Issue number5
DOIs
StatePublished - Dec 1 1998

Fingerprint

chemoattractants
Chemotactic Factors
epidermal growth factor
Epidermal Growth Factor
actin
Actins
Focal Adhesions
Polymerization
polymerization
locomotion
cells
stimulation
Phosphorylation
adenocarcinoma
phosphorylation
cell movement
metastasis
adhesives
Adhesives
breast neoplasms

Keywords

  • Adhesion
  • Cell movement
  • Chemoattractants
  • Chemotaxis
  • Membrane ruffling
  • Protrusion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Anatomy
  • Instrumentation

Cite this

Chemoattractant-induced lamellipod extension. / Bailly, Maryse; Condeelis, John S.; Segall, Jeffrey E.

In: Microscopy Research and Technique, Vol. 43, No. 5, 01.12.1998, p. 433-443.

Research output: Contribution to journalArticle

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