Chemically induced hypothyroidism produces elevated amounts of the α subunit of the inhibitory guanine nucleotide binding protein (G(i)) and the β subunit common to all G-proteins

G. Milligan, Allen M. Spiegel, C. G. Unson, E. D. Saggerson

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36 Citations (Scopus)

Abstract

Adiopocytes of hypothyroid rats display an increased responsiveness to agents which function by inhibiting the production of cyclic AMP. Anti-peptide antisera which selectively recognise the α subunit of the inhibitory guanine nucleotide binding protein (G(i)) detected a 40 kDa polypeptide in adipocyte plasma membranes of both euthyroid and hypothyroid rats. Amounts of the α subunit of G(i) were elevated some 2-fold in the hypothyroid preparations in comparison with the euthyroid controls, when equal amounts of membrane protein of the two treatments were examined. As cells from the hypothyroid animals contained 2.7 times as much membrane protein as those from the control animals, the amounts of α subunit of G(i) are elevated some 5.6-fold per cell in adipocytes of the hypothyroid animals compared with the euthyroid controls. Amounts of the 36 kDa β subunit of G-proteins were also elevated in plasma membranes of adipocytes of hypothyroid animals, in this case by some 50% when compared on a protein basis. These results provide direct evidence for alterations in the amounts of the subunits of G(i) caused by the hypothyroid state.

Original languageEnglish (US)
Pages (from-to)223-227
Number of pages5
JournalBiochemical Journal
Volume247
Issue number1
StatePublished - 1987
Externally publishedYes

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Guanine Nucleotides
Hypothyroidism
GTP-Binding Proteins
Carrier Proteins
Animals
Adipocytes
Cell membranes
Rats
Membrane Proteins
Cell Membrane
Peptides
Cyclic AMP
Immune Sera
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "Chemically induced hypothyroidism produces elevated amounts of the α subunit of the inhibitory guanine nucleotide binding protein (G(i)) and the β subunit common to all G-proteins",
abstract = "Adiopocytes of hypothyroid rats display an increased responsiveness to agents which function by inhibiting the production of cyclic AMP. Anti-peptide antisera which selectively recognise the α subunit of the inhibitory guanine nucleotide binding protein (G(i)) detected a 40 kDa polypeptide in adipocyte plasma membranes of both euthyroid and hypothyroid rats. Amounts of the α subunit of G(i) were elevated some 2-fold in the hypothyroid preparations in comparison with the euthyroid controls, when equal amounts of membrane protein of the two treatments were examined. As cells from the hypothyroid animals contained 2.7 times as much membrane protein as those from the control animals, the amounts of α subunit of G(i) are elevated some 5.6-fold per cell in adipocytes of the hypothyroid animals compared with the euthyroid controls. Amounts of the 36 kDa β subunit of G-proteins were also elevated in plasma membranes of adipocytes of hypothyroid animals, in this case by some 50{\%} when compared on a protein basis. These results provide direct evidence for alterations in the amounts of the subunits of G(i) caused by the hypothyroid state.",
author = "G. Milligan and Spiegel, {Allen M.} and Unson, {C. G.} and Saggerson, {E. D.}",
year = "1987",
language = "English (US)",
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journal = "Biochemical Journal",
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T1 - Chemically induced hypothyroidism produces elevated amounts of the α subunit of the inhibitory guanine nucleotide binding protein (G(i)) and the β subunit common to all G-proteins

AU - Milligan, G.

AU - Spiegel, Allen M.

AU - Unson, C. G.

AU - Saggerson, E. D.

PY - 1987

Y1 - 1987

N2 - Adiopocytes of hypothyroid rats display an increased responsiveness to agents which function by inhibiting the production of cyclic AMP. Anti-peptide antisera which selectively recognise the α subunit of the inhibitory guanine nucleotide binding protein (G(i)) detected a 40 kDa polypeptide in adipocyte plasma membranes of both euthyroid and hypothyroid rats. Amounts of the α subunit of G(i) were elevated some 2-fold in the hypothyroid preparations in comparison with the euthyroid controls, when equal amounts of membrane protein of the two treatments were examined. As cells from the hypothyroid animals contained 2.7 times as much membrane protein as those from the control animals, the amounts of α subunit of G(i) are elevated some 5.6-fold per cell in adipocytes of the hypothyroid animals compared with the euthyroid controls. Amounts of the 36 kDa β subunit of G-proteins were also elevated in plasma membranes of adipocytes of hypothyroid animals, in this case by some 50% when compared on a protein basis. These results provide direct evidence for alterations in the amounts of the subunits of G(i) caused by the hypothyroid state.

AB - Adiopocytes of hypothyroid rats display an increased responsiveness to agents which function by inhibiting the production of cyclic AMP. Anti-peptide antisera which selectively recognise the α subunit of the inhibitory guanine nucleotide binding protein (G(i)) detected a 40 kDa polypeptide in adipocyte plasma membranes of both euthyroid and hypothyroid rats. Amounts of the α subunit of G(i) were elevated some 2-fold in the hypothyroid preparations in comparison with the euthyroid controls, when equal amounts of membrane protein of the two treatments were examined. As cells from the hypothyroid animals contained 2.7 times as much membrane protein as those from the control animals, the amounts of α subunit of G(i) are elevated some 5.6-fold per cell in adipocytes of the hypothyroid animals compared with the euthyroid controls. Amounts of the 36 kDa β subunit of G-proteins were also elevated in plasma membranes of adipocytes of hypothyroid animals, in this case by some 50% when compared on a protein basis. These results provide direct evidence for alterations in the amounts of the subunits of G(i) caused by the hypothyroid state.

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