3′-Deoxy-3′,4′-didehydro-cytidine triphosphate (ddhCTP) is a novel antiviral molecule produced by the enzyme viperin as part of the innate immune response. ddhCTP has been shown to act as an obligate chain terminator of flavivirus and SARS-CoV-2 RNA-dependent RNA polymerases; however, further biophysical studies have been precluded by limited access to this promising antiviral. Herein, we report a robust and scalable synthesis of ddhCTP as well as the mono-and diphosphates ddhCMP and ddhCDP, respectively. Identification of a 2′-silyl ether protection strategy enabled selective synthesis and facile purification of the 5′-Triphosphate, culminating in the preparation of ddhCTP on a gram scale.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Organic Chemistry|
|State||Published - Jul 2 2021|
ASJC Scopus subject areas
- Organic Chemistry