Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface

Claudia J. Morgan; E. Richard Stanley

doi:10.1016/0006-291X(84)91614-0

Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface

Claudia J. Morgan, E. Richard Stanley

Developmental & Molecular Biology

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The cell surface receptor for CSF-1 has been identified by affinity labeling intact mouse bone marrow-derived macrophages and intact cells of the murine J774.2 and BAC1 lines, which differ in their specific CSF-1 binding capacities and biological responses. The receptor, labeled by crosslinking to ¹²⁵I-CSF-1 with disuccinimidyl suberate, is a polypeptide of approximately 165,000 molecular weight, irrespective of cell type.

Original language	English (US)
Pages (from-to)	35-41
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	119
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(84)91614-0
State	Published - Feb 29 1984

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(84)91614-0

Cite this

@article{20e95ab7eb99413ebeeaf285b893c2ae,

title = "Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface",

abstract = "The cell surface receptor for CSF-1 has been identified by affinity labeling intact mouse bone marrow-derived macrophages and intact cells of the murine J774.2 and BAC1 lines, which differ in their specific CSF-1 binding capacities and biological responses. The receptor, labeled by crosslinking to 125I-CSF-1 with disuccinimidyl suberate, is a polypeptide of approximately 165,000 molecular weight, irrespective of cell type.",

author = "Morgan, {Claudia J.} and Stanley, {E. Richard}",

note = "Funding Information: These studies were supported by the National Cancer Institute (CA26504), ahd the AECOM Cancer Center Core Grant. CJM is a postdoctoral fellow supported by NIH training grant 5T32 CA09173. We thank Fran Pellerito excellent technical assistance and Lenore Grollman for help in preparation of the manuscript.",

year = "1984",

month = feb,

day = "29",

doi = "10.1016/0006-291X(84)91614-0",

language = "English (US)",

volume = "119",

pages = "35--41",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface

AU - Morgan, Claudia J.

AU - Stanley, E. Richard

N1 - Funding Information: These studies were supported by the National Cancer Institute (CA26504), ahd the AECOM Cancer Center Core Grant. CJM is a postdoctoral fellow supported by NIH training grant 5T32 CA09173. We thank Fran Pellerito excellent technical assistance and Lenore Grollman for help in preparation of the manuscript.

PY - 1984/2/29

Y1 - 1984/2/29

N2 - The cell surface receptor for CSF-1 has been identified by affinity labeling intact mouse bone marrow-derived macrophages and intact cells of the murine J774.2 and BAC1 lines, which differ in their specific CSF-1 binding capacities and biological responses. The receptor, labeled by crosslinking to 125I-CSF-1 with disuccinimidyl suberate, is a polypeptide of approximately 165,000 molecular weight, irrespective of cell type.

AB - The cell surface receptor for CSF-1 has been identified by affinity labeling intact mouse bone marrow-derived macrophages and intact cells of the murine J774.2 and BAC1 lines, which differ in their specific CSF-1 binding capacities and biological responses. The receptor, labeled by crosslinking to 125I-CSF-1 with disuccinimidyl suberate, is a polypeptide of approximately 165,000 molecular weight, irrespective of cell type.

UR - http://www.scopus.com/inward/record.url?scp=0021344438&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021344438&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(84)91614-0

DO - 10.1016/0006-291X(84)91614-0

M3 - Article

C2 - 6322783

AN - SCOPUS:0021344438

SN - 0006-291X

VL - 119

SP - 35

EP - 41

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this