Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface

Claudia J. Morgan, E. Richard Stanley

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The cell surface receptor for CSF-1 has been identified by affinity labeling intact mouse bone marrow-derived macrophages and intact cells of the murine J774.2 and BAC1 lines, which differ in their specific CSF-1 binding capacities and biological responses. The receptor, labeled by crosslinking to 125I-CSF-1 with disuccinimidyl suberate, is a polypeptide of approximately 165,000 molecular weight, irrespective of cell type.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume119
Issue number1
DOIs
StatePublished - Feb 29 1984

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Macrophage Colony-Stimulating Factor
Cell Surface Receptors
Phagocytes
Crosslinking
Intercellular Signaling Peptides and Proteins
Macrophages
Labeling
Bone
Molecular Weight
Molecular weight
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface. / Morgan, Claudia J.; Stanley, E. Richard.

In: Biochemical and Biophysical Research Communications, Vol. 119, No. 1, 29.02.1984, p. 35-41.

Research output: Contribution to journalArticle

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