CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo

Alexander Y. Konev, Martin Tribus, Yeon Park Sung, Valerie Podhraski, Yan Lim Chin, Alexander Emelyanov, Elena Vershilova, Vincenzo Pirrotta, James T. Kadonaga, Alexandra Lusser, Dmitry Fyodorov

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

The organization of chromatin affects all aspects of nuclear DNA metabolism in eukaryotes. H3.3 is an evolutionarily conserved histone variant and a key substrate for replication-independent chromatin assembly. Elimination of chromatin remodeling factor CHD1 in Drosophila embryos abolishes incorporation of H3.3 into the male pronucleus, renders the paternal genome unable to participate in zygotic mitoses, and leads to the development of haploid embryos. Furthermore, CHD1, but not ISWI, interacts with HIRA in cytoplasmic extracts. Our findings establish CHD1 as a major factor in replacement histone metabolism in the nucleus and reveal a critical role for CHD1 in the earliest developmental instances of genome-scale, replication-independent nucleosome assembly. Furthermore, our results point to the general requirement of adenosine triphosphate (ATP) - utilizing motor proteins for histone deposition in vivo.

Original languageEnglish (US)
Pages (from-to)1087-1090
Number of pages4
JournalScience
Volume317
Issue number5841
DOIs
StatePublished - Aug 24 2007

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Histones
Chromatin
Chromatin Assembly and Disassembly
Genome
Proteins
Nucleosomes
Haploidy
Eukaryota
Mitosis
Drosophila
Embryonic Development
Embryonic Structures
Adenosine Triphosphate
DNA

ASJC Scopus subject areas

  • General

Cite this

CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo. / Konev, Alexander Y.; Tribus, Martin; Sung, Yeon Park; Podhraski, Valerie; Chin, Yan Lim; Emelyanov, Alexander; Vershilova, Elena; Pirrotta, Vincenzo; Kadonaga, James T.; Lusser, Alexandra; Fyodorov, Dmitry.

In: Science, Vol. 317, No. 5841, 24.08.2007, p. 1087-1090.

Research output: Contribution to journalArticle

Konev, AY, Tribus, M, Sung, YP, Podhraski, V, Chin, YL, Emelyanov, A, Vershilova, E, Pirrotta, V, Kadonaga, JT, Lusser, A & Fyodorov, D 2007, 'CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo', Science, vol. 317, no. 5841, pp. 1087-1090. https://doi.org/10.1126/science.1145339
Konev, Alexander Y. ; Tribus, Martin ; Sung, Yeon Park ; Podhraski, Valerie ; Chin, Yan Lim ; Emelyanov, Alexander ; Vershilova, Elena ; Pirrotta, Vincenzo ; Kadonaga, James T. ; Lusser, Alexandra ; Fyodorov, Dmitry. / CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo. In: Science. 2007 ; Vol. 317, No. 5841. pp. 1087-1090.
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