Characterization of Trypanosoma cruzi infectivity, proliferation, and cytokine patterns in gut and pancreatic epithelial cells maintained in vitro

Laura A. Martello, Raj Wadgaonkar, Raavi Gupta, Fabiana S. Machado, Michael G. Walsh, Eduardo Mascareno, Herbert B. Tanowitz, M. A. Haseeb

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Trypanosoma cruzi infects all nucleated cells in both humans and experimental animals. As a prelude to our studies of T. cruzi pathogenesis in the gastrointestinal system, we have initiated in vitro cultures of gut (Caco-2 and HT-29) and pancreatic (Panc-1) epithelial cells. We show that along with primary human fibroblasts, all three cell lines are susceptible to infection and support proliferation of T. cruzi. Infection with T. cruzi modified dramatically the cytokines elaborated by these cells. Substantially greater quantities of IL-5 and TGF-β1 were produced by fibroblasts and Caco-2 and Panc-1 cells, whereas secretion of IFN-γ and TNF-α was greatly reduced in all three cell types. Since these cells are not known to be the primary sources of IFN-γ, we examined IFN-γ mRNA expression in these cells. Both Caco-2 and Panc-1 cells were found to express IFN-γ mRNA, validating its secretion. These findings may provide insight into signaling pathways that mediate innate immunity to T. cruzi and pathogenesis of gastrointestinal and pancreatic alterations in Chagas disease.

Original languageEnglish (US)
Pages (from-to)4177-4183
Number of pages7
JournalParasitology Research
Volume112
Issue number12
DOIs
StatePublished - Dec 2013

Fingerprint

Trypanosoma cruzi
epithelial cells
cytokines
pathogenicity
digestive system
Epithelial Cells
Cytokines
cells
fibroblasts
pathogenesis
Fibroblasts
secretion
interleukin-5
Messenger RNA
Chagas disease
Chagas Disease
biological resistance
Interleukin-5
Infection
In Vitro Techniques

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Martello, L. A., Wadgaonkar, R., Gupta, R., Machado, F. S., Walsh, M. G., Mascareno, E., ... Haseeb, M. A. (2013). Characterization of Trypanosoma cruzi infectivity, proliferation, and cytokine patterns in gut and pancreatic epithelial cells maintained in vitro. Parasitology Research, 112(12), 4177-4183. https://doi.org/10.1007/s00436-013-3609-7

Characterization of Trypanosoma cruzi infectivity, proliferation, and cytokine patterns in gut and pancreatic epithelial cells maintained in vitro. / Martello, Laura A.; Wadgaonkar, Raj; Gupta, Raavi; Machado, Fabiana S.; Walsh, Michael G.; Mascareno, Eduardo; Tanowitz, Herbert B.; Haseeb, M. A.

In: Parasitology Research, Vol. 112, No. 12, 12.2013, p. 4177-4183.

Research output: Contribution to journalArticle

Martello, LA, Wadgaonkar, R, Gupta, R, Machado, FS, Walsh, MG, Mascareno, E, Tanowitz, HB & Haseeb, MA 2013, 'Characterization of Trypanosoma cruzi infectivity, proliferation, and cytokine patterns in gut and pancreatic epithelial cells maintained in vitro', Parasitology Research, vol. 112, no. 12, pp. 4177-4183. https://doi.org/10.1007/s00436-013-3609-7
Martello, Laura A. ; Wadgaonkar, Raj ; Gupta, Raavi ; Machado, Fabiana S. ; Walsh, Michael G. ; Mascareno, Eduardo ; Tanowitz, Herbert B. ; Haseeb, M. A. / Characterization of Trypanosoma cruzi infectivity, proliferation, and cytokine patterns in gut and pancreatic epithelial cells maintained in vitro. In: Parasitology Research. 2013 ; Vol. 112, No. 12. pp. 4177-4183.
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