Characterization of TRIM5α trimerization and its contribution to human immunodeficiency virus capsid binding

Hassan Javanbakht, Wen Yuan, Darwin F. Yeung, Byeongwoon Song, Felipe Diaz-Griffero, Yuan Li, Xing Li, Matthew Stremlau, Joseph Sodroski

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The coiled-coil domain of the tripartite motif (TRIM) family protein TRIM5α is required for trimerization and function as an antiretroviral restriction factor. Unlike the coiled-coil regions of other related TRIM proteins, the coiled coil of TRIM5α is not sufficient for multimerization. The linker region between the coiled-coil and B30.2 domains is necessary for efficient TRIM5α trimerization. Most of the hydrophilic residues predicted to be located on the surface-exposed face of the coiled coil can be altered without compromising TRIM5α antiviral activity against human immunodeficiency virus (HIV-1). However, changes that disrupt TRIM5α trimerization proportionately affect the ability of TRIM5α to bind HIV-1 capsid complexes. Therefore, TRIM5α trimerization makes a major contribution to its avidity for the retroviral capsid, and to the ability to restrict virus infection.

Original languageEnglish (US)
Pages (from-to)234-246
Number of pages13
JournalVirology
Volume353
Issue number1
DOIs
StatePublished - Sep 15 2006
Externally publishedYes

Fingerprint

Virus Attachment
Capsid
HIV-1
HIV
Virus Diseases
Antiviral Agents
Tripartite Motif Proteins

Keywords

  • Coiled-coil domain
  • HIV-1
  • Retroviral capsid
  • TRIM5α
  • Trimerization

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Characterization of TRIM5α trimerization and its contribution to human immunodeficiency virus capsid binding. / Javanbakht, Hassan; Yuan, Wen; Yeung, Darwin F.; Song, Byeongwoon; Diaz-Griffero, Felipe; Li, Yuan; Li, Xing; Stremlau, Matthew; Sodroski, Joseph.

In: Virology, Vol. 353, No. 1, 15.09.2006, p. 234-246.

Research output: Contribution to journalArticle

Javanbakht, H, Yuan, W, Yeung, DF, Song, B, Diaz-Griffero, F, Li, Y, Li, X, Stremlau, M & Sodroski, J 2006, 'Characterization of TRIM5α trimerization and its contribution to human immunodeficiency virus capsid binding', Virology, vol. 353, no. 1, pp. 234-246. https://doi.org/10.1016/j.virol.2006.05.017
Javanbakht, Hassan ; Yuan, Wen ; Yeung, Darwin F. ; Song, Byeongwoon ; Diaz-Griffero, Felipe ; Li, Yuan ; Li, Xing ; Stremlau, Matthew ; Sodroski, Joseph. / Characterization of TRIM5α trimerization and its contribution to human immunodeficiency virus capsid binding. In: Virology. 2006 ; Vol. 353, No. 1. pp. 234-246.
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