Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase

James Mele, Holly Van Remmen, Jan Vijg, Arlan Richardson

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Transgenic mice overexpressing both Cu/ZnSOD and catalase [Tg(SOD1/CAT)+/0] were used to evaluate the effects of overexpression of both genes against oxidative stress. Characterization of these transgenic mice revealed that catalase or Cu/ZnSOD activities were two- to fourfold higher in the tissues of transgenic mice compared to wild-type mice, and the activities of the other major antioxidant enzymes were not altered in the tissues of the transgenic mice. The murine embryonic fibroblasts (MEFs) from the Tg(SOD1/CAT)+/0 and MEFs overexpressing Cu/ZnSOD were more resistant to paraquat cytotoxicity, relative to wild-type MEFs. The MEFs from Tg(SOD1/CAT)+/0 tended to be more resistant (up to 2.25-fold) to paraquat cytotoxicity than MEFs overexpressing either Cu/ZnSOD or catalase alone. MEFs from Tg(CAT)+/0 and Tg(SOD1/CAT)+/0 were equally as resistant to hydrogen peroxide cytotoxicity. However, there were no significant differences in whole animal survival against either paraquat or γ-radiation.

Original languageEnglish (US)
Pages (from-to)628-638
Number of pages11
JournalAntioxidants and Redox Signaling
Volume8
Issue number3-4
DOIs
StatePublished - Mar 2006
Externally publishedYes

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Fibroblasts
Catalase
Transgenic Mice
Superoxide Dismutase
Zinc
Copper
Paraquat
Cytotoxicity
Tissue
Oxidative stress
Hydrogen Peroxide
Animals
Oxidative Stress
Antioxidants
Genes
Radiation
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase. / Mele, James; Van Remmen, Holly; Vijg, Jan; Richardson, Arlan.

In: Antioxidants and Redox Signaling, Vol. 8, No. 3-4, 03.2006, p. 628-638.

Research output: Contribution to journalArticle

Mele, James ; Van Remmen, Holly ; Vijg, Jan ; Richardson, Arlan. / Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase. In: Antioxidants and Redox Signaling. 2006 ; Vol. 8, No. 3-4. pp. 628-638.
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