Abstract
Transgenic mice overexpressing both Cu/ZnSOD and catalase [Tg(SOD1/CAT)+/0] were used to evaluate the effects of overexpression of both genes against oxidative stress. Characterization of these transgenic mice revealed that catalase or Cu/ZnSOD activities were two- to fourfold higher in the tissues of transgenic mice compared to wild-type mice, and the activities of the other major antioxidant enzymes were not altered in the tissues of the transgenic mice. The murine embryonic fibroblasts (MEFs) from the Tg(SOD1/CAT)+/0 and MEFs overexpressing Cu/ZnSOD were more resistant to paraquat cytotoxicity, relative to wild-type MEFs. The MEFs from Tg(SOD1/CAT)+/0 tended to be more resistant (up to 2.25-fold) to paraquat cytotoxicity than MEFs overexpressing either Cu/ZnSOD or catalase alone. MEFs from Tg(CAT)+/0 and Tg(SOD1/CAT)+/0 were equally as resistant to hydrogen peroxide cytotoxicity. However, there were no significant differences in whole animal survival against either paraquat or γ-radiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 628-638 |
| Number of pages | 11 |
| Journal | Antioxidants and Redox Signaling |
| Volume | 8 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - Mar 2006 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Molecular Biology
- Clinical Biochemistry
- Cell Biology