Abstract
The N-linked glycans of murine mAb 12A1 were characterized by LC/MS/MS coupled with Glu-C digestion in denature condition. The 12A1 were reduced and carboxyamidomethylated in 6 M GdHCl and 0.25 M phosphate buffer. Glu-C digestion was performed. The digestion was separated by micro HPLC and detected by the quadrupole ion trap mass spectrometer. The oligosaccharide chains of this antibody were characterized at N glycosylation site of Asn-363.
| Original language | English (US) |
|---|---|
| Pages | 469-470 |
| Number of pages | 2 |
| State | Published - 2002 |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
| Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country/Territory | United States |
| City | Orlando, FL |
| Period | 6/2/02 → 6/6/02 |
ASJC Scopus subject areas
- Spectroscopy