Abstract
The N-linked glycans of murine mAb 12A1 were characterized by LC/MS/MS coupled with Glu-C digestion in denature condition. The 12A1 were reduced and carboxyamidomethylated in 6 M GdHCl and 0.25 M phosphate buffer. Glu-C digestion was performed. The digestion was separated by micro HPLC and detected by the quadrupole ion trap mass spectrometer. The oligosaccharide chains of this antibody were characterized at N glycosylation site of Asn-363.
Original language | English (US) |
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Pages | 469-470 |
Number of pages | 2 |
State | Published - 2002 |
Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
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Country/Territory | United States |
City | Orlando, FL |
Period | 6/2/02 → 6/6/02 |
ASJC Scopus subject areas
- Spectroscopy