Characterization of the N-linked glycans of a murine monoclonal antibody 12A1 by LC/MS/MS

Fang Wang, Antonio Nakouzi, Ruth H. Angeletti, Arturo Casadevall

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The N-linked glycans of murine mAb 12A1 were characterized by LC/MS/MS coupled with Glu-C digestion in denature condition. The 12A1 were reduced and carboxyamidomethylated in 6 M GdHCl and 0.25 M phosphate buffer. Glu-C digestion was performed. The digestion was separated by micro HPLC and detected by the quadrupole ion trap mass spectrometer. The oligosaccharide chains of this antibody were characterized at N glycosylation site of Asn-363.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages469-470
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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Glycosylation
Mass spectrometers
Oligosaccharides
Polysaccharides
Buffers
Phosphates
Monoclonal Antibodies
Ions
Antibodies

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Wang, F., Nakouzi, A., Angeletti, R. H., & Casadevall, A. (2002). Characterization of the N-linked glycans of a murine monoclonal antibody 12A1 by LC/MS/MS. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 469-470)

Characterization of the N-linked glycans of a murine monoclonal antibody 12A1 by LC/MS/MS. / Wang, Fang; Nakouzi, Antonio; Angeletti, Ruth H.; Casadevall, Arturo.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 469-470.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, F, Nakouzi, A, Angeletti, RH & Casadevall, A 2002, Characterization of the N-linked glycans of a murine monoclonal antibody 12A1 by LC/MS/MS. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 469-470, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Wang F, Nakouzi A, Angeletti RH, Casadevall A. Characterization of the N-linked glycans of a murine monoclonal antibody 12A1 by LC/MS/MS. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 469-470
Wang, Fang ; Nakouzi, Antonio ; Angeletti, Ruth H. ; Casadevall, Arturo. / Characterization of the N-linked glycans of a murine monoclonal antibody 12A1 by LC/MS/MS. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 469-470
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