Characterization of somatically mutated S107 VH11-encoded anti-DNA autoantibodies derived from autoimmune (NZB × NZW)F1 mice

Samuel M. Behar, Daniel L. Lustgarten, Sylvie Corbet, Matthew D. Scharff

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

We have studied 19 S107 heavy chain variable region gene (VH11)-encoded monoclonal antibodies from NZBWF1 mice. These studies show that a single VH gene can encode both antibodies to foreign antigens (anti-phosphorylcholine) and to self antigens (anti-double-stranded DNA) in the same animal. All of the anti-DNA antibodies contain many somatic mutations compared with the relevant germline genes. Since the anti-DNA antibodies were extensively somatically mutated and had undergone isotype switching, the response seems to be T cell dependent. While some of the antibodies appear to be the products of an antigen-driven and antigen-selected response, a number of characteristics of the antibodies suggest that forces other than antigen are contributing to the stimulation and selection of this response.

Original languageEnglish (US)
Pages (from-to)731-741
Number of pages11
JournalJournal of Experimental Medicine
Volume173
Issue number3
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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