Characterization of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5)

Kausik Si, Kallol Das, Umadas Maitra

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Eukaryotic translation initiation factor 5 (eIF-5) interacts with the 40 S initiation complex (40S · mRNA · Met-tRNA(f) · eIF-2 · GTP) to promote the hydrolysis of bound GTP with the concomitant joining of the 60 S ribosomal subunit to the 40 S initiation complex to form a functional 80 S initiation complex. In this paper, the multiple mRNAs that encode mammalian eIF-5 have been characterized. In rat tissues, three major eIF-5 mRNAs of 3.5, 2.8, and 2.2 kilobases in length are detected. All major eIF-5 mRNAs are initiated from a single transcription initiation site, contain identical 5'- untranslated and coding regions, but differ from one another only in the length of their 3'-untranslated regions. The different lengths of the 3'- untranslated region of eIF-5 mRNAs are generated by the use of alternative polyadenylation signals. Additionally, we demonstrate tissue-specific variations in eIF-5 mRNA expression as well as preference for polyadenylation sites. These results should lead to increased understanding of the regulation of eIF-5 gene expression.

Original languageEnglish (US)
Pages (from-to)16934-16938
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number28
DOIs
StatePublished - 1996

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Peptide Initiation Factors
Messenger RNA
Polyadenylation
3' Untranslated Regions
Guanosine Triphosphate
Eukaryotic Initiation Factor-5
Tissue
Eukaryotic Initiation Factors
Ribosome Subunits
5' Untranslated Regions
Transcription Initiation Site
Gene expression
Joining
Rats
Hydrolysis
Gene Expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5). / Si, Kausik; Das, Kallol; Maitra, Umadas.

In: Journal of Biological Chemistry, Vol. 271, No. 28, 1996, p. 16934-16938.

Research output: Contribution to journalArticle

Si, Kausik ; Das, Kallol ; Maitra, Umadas. / Characterization of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5). In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 28. pp. 16934-16938.
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