Characterization of multiple mRNAs that encode mammalian translation initiation factor 5 (eIF-5)

Kausik Si, Kallol Das, Umadas Maitra

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Eukaryotic translation initiation factor 5 (eIF-5) interacts with the 40 S initiation complex (40S · mRNA · Met-tRNA(f) · eIF-2 · GTP) to promote the hydrolysis of bound GTP with the concomitant joining of the 60 S ribosomal subunit to the 40 S initiation complex to form a functional 80 S initiation complex. In this paper, the multiple mRNAs that encode mammalian eIF-5 have been characterized. In rat tissues, three major eIF-5 mRNAs of 3.5, 2.8, and 2.2 kilobases in length are detected. All major eIF-5 mRNAs are initiated from a single transcription initiation site, contain identical 5'- untranslated and coding regions, but differ from one another only in the length of their 3'-untranslated regions. The different lengths of the 3'- untranslated region of eIF-5 mRNAs are generated by the use of alternative polyadenylation signals. Additionally, we demonstrate tissue-specific variations in eIF-5 mRNA expression as well as preference for polyadenylation sites. These results should lead to increased understanding of the regulation of eIF-5 gene expression.

Original languageEnglish (US)
Pages (from-to)16934-16938
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number28
DOIs
StatePublished - Jul 30 1996

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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